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The citric acid cycle enzyme malate dehydrogenase was purified to homogeneity from the nonsulfur purple bacteria Rhodobacter capsulatus, Rhodospirillum rubrum, Rhodomicrobium vannielii, and Rhodocyclus purpureus. Malate dehydrogenase was purified from each species by either a single- or a two-step protocol: triazine dye affinity chromatography was the key(More)
Purified malate dehydrogenases from four species of non-sulphur purple phototrophic bacteria were examined for their heat-stability, amino acid composition and antigenic relationships. Malate dehydrogenase from Rhodospirillum rubrum, Rhodobacter capsulatus and Rhodomicrobium vannielii (which are all tetrameric proteins) had an unusually high glycine(More)
Motivation Im Requirements Engineering Umfeld, gibt es seit längerem den Leitsatz alle seine Anforderungen positiv zu formulieren. Im Grunde naheliegend, denn schließlich beschreibt eine Spezifikation ja das Produkt das man erwartet und nicht das Produkt welches man nicht haben möchte. Dieser Leitsatz manifestiert sich z.B. im vielzitiertem Standard IEEE(More)
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