Maksimiljan Sterle

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Using primary explant cultures of mouse bladder, the early response of the urothelium after superficial and full-thickness injuries was investigated. In such an in vitro wound healing model, explant surfaces with a mostly desquamated urothelial superficial layer represented superficial wounds, and the exposed lamina propria at the cut edges of the explants(More)
The purpose of this study was to establish an in vitro culture model that closely resembles whole mouse urothelial tissue. Primary explant cultures of mouse bladder were established on porous membrane supports and explant outgrowths were analysed for morphology and the presence of antigenic and ultrastructural markers associated with urothelial(More)
The activity of mitochondrial cytochrome oxidase and peroxisomal catalase in the phagolysosomes and apoptotic bodies of mucoid epithelial cells was analysed. Tissue from 2–6 day old mice was used. The activity of acid phosphatase in lysosomes was also estimated. Cytochrome oxidase was demonstrated in well-preserved mitochondria inside phagosomes.(More)
The effects of two growth factors, EGF and TGF beta 1, on growth and differentiation of different populations of urothelial cells in explant cultures of mouse urinary bladder have been studied by electron microscopy and lectin analysis. In an explant culture 10 days after the implantation three different populations of urothelial cells can be distinguished.(More)
In this study, we report a reliable technique for the harvest, cultivation and expansion of monoculture of NMU. The NMU were harvested by two methods, directly from the urothelium in vivo and indirectly from the urothelial outgrowths of bladder explant cultures. Primary cultures and subsequent subcultures were propagated in the mixture of media MCDB 153 and(More)
Leading edge cells, which are located at the forefront of a wound margin, play a significant role in coordinating the wound healing process. In this study, leading edge cells of the urothelial explant outgrowth, resembling leading edge cells during urothelial full-thickness wound healing in vivo, were analyzed for expression and distribution of junction and(More)
The origin of late endosomes - multivesicular bodies (MVBs) in the superficial cells of 16 and 17 embryonic old transitional epithelium of mouse urinary bladder was studied by electron microscopy, lectin labelling and HRP tracing. Analysis of hexagonally structured membrane particles, WGA, and RCA I binding sites revealed structural similarity between(More)
In developing mouse urinary bladder the urothelial cells respond to urine accumulation by cell detachment, i.e. desquamation. To elucidate the steps of urothelial cell detachment during embryonic development and first urine accumulation in bladder lumen, superficial and intermediate cell layers were investigated. Different electron microscopic and(More)
To elucidate the effects of epidermal growth factor-EGF and transforming growth factor-TGFβ1 on cellular structure, especially on cell junctions and cytoskeleton, the distribution of ZO1, E-cadherin and desmoplakin as well as the organization of actin and keratin filaments have been examined immunohistochemically. In EGF-treated cultures as well as in(More)
The effect of lamina propria on the growth and differentiation of the mouse urinary bladder urothelial cells in vitro has been studied by light and electron microscopy using morphological and immunohistochemical methods. Three different types of urothelial cultures were maintained on a porous membrane in serum-free medium for 10 days. Our results showed(More)