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CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L.
TLDR
The application of CRISPR/Cas9 to targeted mutagenesis in the liverwort Marchantia polymorpha L., which has emerged as a model species for studying land plant evolution, provides a rapid and simple approach for molecular genetics in M.polymorpha, and raises the possibility that the system may be applied to a wide variety of plant species. Expand
Vacuolar SNAREs function in the formation of the leaf vascular network by regulating auxin distribution.
TLDR
It is shown that vacuolar SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), VAM3 and VTI11, are required for the formation of the leaf vascular network in a dosage-dependent manner and that PIN1 proteins were constitutively transported to vacuoles in leaf and roots cells. Expand
Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome
TLDR
Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. Expand
The AP-1 μ adaptin is required for KNOLLE localization at the cell plate to mediate cytokinesis in Arabidopsis.
TLDR
It is proposed that the AP-1 complex is required for cell plate-targeted trafficking of KN in dividing plant cells, and that it has a common role in mediating plant and yeast/animal cytokinesis systems which are fundamentally different. Expand
Identification and Dynamics of Arabidopsis Adaptor Protein-2 Complex and Its Involvement in Floral Organ Development[W]
TLDR
The study provides a molecular basis for understanding AP-2–dependent endocytic pathways in plants and their roles in floral organ development and plant reproduction. Expand
The Adaptor Complex AP-4 Regulates Vacuolar Protein Sorting at the trans-Golgi Network by Interacting with VACUOLAR SORTING RECEPTOR11
TLDR
A novel function for the AP-4 complex in VSR1-mediated vacuolar protein sorting at the specialized domain of the TGN is provided. Expand
Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development.
TLDR
SyP2 proteins, including cytosolic SYP23/PLP, appear to function redundantly in vacuolar trafficking and plant development and it is found that expression of multiple copies of a genomic fragment of SYP21/pep12 suppressed the abnormal syp22-3 phenotype. Expand
Transcriptional Framework of Male Gametogenesis in the Liverwort Marchantia polymorpha L.
TLDR
This study provides a transcriptional framework on which to study the molecular mechanism of plant motile sperm development in M. polymorpha as a model and shows that some of them had distinct expression patterns in the spermatogenous tissue of developing antheridia. Expand
MAG2 and three MAG2-INTERACTING PROTEINs form an ER-localized complex to facilitate storage protein transport in Arabidopsis thaliana.
TLDR
The results suggest that MAG2 and the three MIP proteins form a unique complex on the ER that is responsible for efficient transport of seed storage proteins. Expand
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