Makoto Matsuzawa

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A field-effect transistor (FET) array has been fabricated and used for recording of electrical signals from neural cells. The array consists of p-channel FETs with non-metalized gates. The size of the gates of the 16 FETs are from 28 x 12 microns2 down to 10 x 4 microns2 and are arranged in a 4 x 4 matrix on 200 microns centers. For the device fabrication(More)
We have studied the electrophysiological properties of hippocampal neurons grown on surfaces of organic thin films formed on glass or silicon substrates and on microelectronic device surfaces in culture. Hippocampal neurons were dissociated from embryonic rats and plated on substrates chemically modified with laminin peptide in a chemically defined medium.(More)
We show that axon guidance of embryonic hippocampal neurons is promoted by pathways of a decapeptide (RDIAEIIKDI) derived from a neurite outgrowth domain of the gamma1 chain of laminin-1. This guidance is directly dependent on: (1) a concentration difference of the decapeptide between the peptide pathway and its surrounding areas, and (2) the optimal(More)
The use of geometrically simple networks formed by cultured neurons facilitates the electrophysiological study of biological computation. We used chemically patterned substrates for culturing SK-N-SH human neuroblastoma cells and embryonic rat hippocampal neurons to geometrically control their neurite outgrowth. On patterned substrates (parallel lines, 5-10(More)
We describe here a modification procedure for chemically fabricating neuron adhesive substrates to study the substratum-guided neurite outgrowth in culture. These substrates were fabricated by chemically attaching a synthetic peptide derived from a neurite-out-growth-promoting domain of the B2 chain of laminin. The attachment was carried out by coupling the(More)
Using a small-angle X-ray scattering technique, shape and size, and internal structure of diglycerol polyisostearate nonionic surfactant micelles in nonpolar oil n-hexadecane (HD) were investigated at 25 degrees C. Furthermore, the effect of added water on the structure of host reverse micelles was also investigated. The scattering data were evaluated by(More)
We used ordered arrangements of neuroblastoma cells in culture on chemically patterned substrates to direct the orientation of electrical stimulation with respect to cell alignment. Chemically patterned parallel lines of self-assembled monolayer films were fabricated on glass substrates via a deep UV lithographic procedure. Cultured neuroblastoma cells(More)
Patterned substrates offer the promise of controlled positioning and directional guidance of growing neurites. Therefore, they could be useful for constructing small neuronal networks with defined geometry in vitro. We have fabricated chemically patterned substrates using self-assembled monolayer films with a lithographic mask technique and demonstrated the(More)
We created a new culture system which provides simple, stereotyped neuronal circuitries suitable for investigating synaptic events between mammalian central neurons. We used surface chemistry and laser-lithography to produce geometrical patterns of neuron-compatible substrate spaced by less neuron-compatible surfaces. The patterned substrates were composed(More)
The structure of glycerol-based reverse micelles in the surfactant/oil binary system without external water addition has been investigated using a small-angle X-ray scattering technique, and different tunable parameters for the structure control of reverse micelles are determined. The scattering data were evaluated by the generalized indirect Fourier(More)