Makiko Ohtani

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OBJECTIVE To perform a trial to assess the efficacy of radical scavengers, i.e. rebamipide, vitamin C and glutathione, for the treatment of Ménière's disease (MD). MATERIAL AND METHODS Rebamipide (300 mg/day), vitamin C (600 mg/day) and/or glutathione (300 mg/day) were given orally for at least 8 weeks to 25 patients with poorly controlled MD. RESULTS(More)
CONCLUSION The results of this study indicate that transient receptor potential subfamily 1 (TRPV1) may play a functional role in sensory cell physiology and that TRPV4 may be important for fluid homeostasis in the inner ear. OBJECTIVE To analyze the expression of TRPV1 and -4 in the normal guinea pig inner ear. MATERIAL AND METHODS Albino guinea pigs(More)
Caloric responses to intracellular calcium homeostasis of vestibular hair cells isolated from the guinea pig were examined. An increase in intracellular free calcium ion concentration ([Ca2+]i) in the isolated cells was observed by direct heat stimulation. The cells, dissociated enzymatically or mechanically, were stimulated by perfusion of solution, in the(More)
Single, viable taste receptor cells were isolated from the tongue of the guinea pig by enzymatic digestion and mechanical dissociation. The cells could be classified into flask, spindle and intermediate shapes. The intracellular free calcium ion concentrations [(Ca2+)i] of these cells were determined using the Ca2+ sensitive dye fura-2 and digital imaging(More)
Single inner hair cells of the guinea pig cochlea were isolated using enzymatic and mechanical techniques. The intracellular free calcium ion concentrations [( Ca2+]i) of the isolated inner hair cells were determined using the Ca2+ sensitive dye fura-2 and digital imaging microscopy. In the presence of 1 micron ionomycin, a Ca2+ ionophore, there was an(More)
The effects of putative efferent neurotransmitters on intracellular Ca2+ concentration ([Ca2+]i) in isolated vestibular hair cells (VHCs) of the guinea pig were determined using the Ca2+ sensitive dye Fura-2 and digital imaging microscopy. In the presence of 1 mM acetylcholine (ACh) there was a gradual increase in [Ca2+]i. In the presence of 10 microM ATP(More)
Vestibular hair cells were isolated from the macula utriculi, macula sacculi and crista ampullaris of the guinea pig, using enzymatic and mechanical techniques. The cells could be classified into two types: flask-shaped ones with a neck and rod- or round-shaped ones without a neck. The flask-shaped cells were thought to be type I cells, the rod-shaped cells(More)
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