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Structural conversion of neurotoxic amyloid-β(1–42) oligomers to fibrils
TLDR
It is shown that low-temperature and low-salt conditions can stabilize disc-shaped oligomers (pentamers) that are substantially more toxic to mouse cortical neurons than protofibrils and fibrils, and that these neurotoxic oligomers do not have the β-sheet structure characteristic of fibril. Expand
High-resolution atomic force microscopy of soluble Abeta42 oligomers.
TLDR
The observation of fine structure in the high MW oligomers suggests that they are able to nucleate fibril formation, and it is shown that an inhibitor designed to block the sheet-to-sheet packing in Abeta fibrils is able to cap the heights of these oligomers at approximately 4 nm. Expand
Fusion of Cells by Flipped SNAREs
TLDR
Cells expressing the interacting domains of v- and t-SNAREs on the cell surface were found to fuse spontaneously, demonstrating that SNAREs are sufficient to fuse biological membranes. Expand
FKBP12 binds to acylated H-ras and promotes depalmitoylation.
TLDR
It is demonstrated that FKBP12 regulates H-Ras trafficking by promoting depalmitoylation through cis-trans isomerization of a peptidyl-prolyl bond in proximity to the palmitoylated cysteines. Expand
Inhibitors of amyloid toxicity based on beta-sheet packing of Abeta40 and Abeta42.
TLDR
It is shown that peptide inhibitors based on a GxFxGxF framework disrupt sheet-to-sheet packing and inhibit the formation of mature Abeta fibrils as assayed by thioflavin T fluorescence, electron microscopy, and solid-state NMR spectroscopy. Expand
Degradation of Amyloid β Protein by Purified Myelin Basic Protein*
TLDR
It is shown that Aβ40 and Aβ42 peptides are degraded by purified human brain MBP and recombinant human MBP, but not an MBP fragment that lacks autolytic activity, which indicates that purified MBP possesses Aβ degrading activity in vitro. Expand
Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage.
TLDR
Results of extensive cross-linking and affinity cleavage experiments using single-cysteine P protein variants derivatized with either azidophenacyl bromide or 5-iodoacetamido-1,10-o-phenanthroline to determine distance constraints and to model the Bacillus subtilis holoenzyme-substrate complex indicate that P protein is positioned close to the RNase P active site and may play a role in organizing the RN enzyme. Expand
Engineering anti‐GD2 monoclonal antibodies for cancer immunotherapy
TLDR
To enhance anti‐tumor efficacy, anti‐GD2 monoclonal antibodies and fragments have been engineered into immunocytokines, immunotoxins, antibody drug conjugates, radiolabeled antibodies, targeted nanoparticles, T‐cell engaging bispecific antibodies, and chimeric antigen receptors. Expand
N-terminal Domain of Myelin Basic Protein Inhibits Amyloid β-Protein Fibril Assembly*
TLDR
It is demonstrated that human MBP purified from either brain or a bacterial recombinant expression system comparably bound to Aβ and inhibited Aβ fibril assembly, indicating that post-translational modifications are not required for this activity. Expand
Meditation as a Potential Therapy for Autism: A Review
TLDR
Evidence from clinical studies and neuroscience research is summarized that suggests that an approach built on yogic principles and meditative tools is worth pursuing, and desired outcomes include relief of clinical symptoms of the disease, greater relaxation, and facilitated expression of feelings and skills, as well as improved family and social quality of life. Expand
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