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A simple, accurate and selective HPLC method was developed and validated for determination of quercetin and kaempferol, which are the main flavonols in broccoli. The separation was achieved on a reversed-phase C18 column using a mobile phase composed of methanol/water (60/40) and phosphoric acid 0.2% at a flow rate of 1.0 ml·min. The detection was carried(More)
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