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The body wall muscles in five species of branchiobdellidans are all arranged in the oligochaete pattern and the muscle fibres are obliquely striated. The structure of the circular muscle fibres do vary to some degree. The longitudinal muscle fibres in Ankyrodrilus legaeus, Branchiobdella kozarovi, and Xironogiton instabilis all are round circomyarian and(More)
We have analyzed midgut development during the fifth larval instar in the tobacco budworm Heliothis virescens. In prepupae, the midgut formed during larval instars undergoes a complete renewal process. This drastic remodeling of the alimentary canal involves the destruction of the old cells by programmed cell-death mechanisms (autophagy and apoptosis).(More)
An increasing body of evidence indicates that in the leech Hirudo medicinalis the angiogenic process is finely regulated and coordinated by the botryoidal tissue. In this paper we provide evidence on the involvement of botryoidal tissue cells in angiogenesis induced in H. medicinalis by a variety of stimuli including surgical wounds or the administration of(More)
The fine structure of leech body wall muscles, has been analysed under great length variations. All the measurable parameters of the fibre (distance between thick filaments, sarcomere and A band width, percentage of actin crowns around myosin filaments, fibre radius) at different elongations were tested with equations describing the geometrical model of(More)
Functional and morphological modifications in the IPLB-LdFB insect cell line were examined following a short treatment with a reversible inhibitor of mitochondrial ATP synthase, oligomycin A, and subsequent incubation for various times in oligomycin-A-free medium. Oncosis, apoptosis and autophagy at variable percentages were observed under the various(More)
Human parthenogenetic embryos have recently been proposed as an alternative, less controversial source of embryonic stem cell (ESC) lines; however many aspects related to the biology of parthenogenetic embryos and parthenogenetic derived cell lines still need to be elucidated. We present here results on human cell lines (HP1 and HP3) derived from(More)
Parthenotes have been proposed as a source of embryonic stem cells but they lack the centriole which is inherited through the sperm in all mammalian species, except for rodents. We investigated the centrosome of parthenotes and parthenogenetic embryonic stem cells using parthenogenetic and biparental pig pre-implantation embryos, human and pig(More)
Longitudinal body wall muscles of lumbriculids are characterized by unique peculiarities; they are ribbonshaped, with regularly distributed Z-elements and sarcotubules crossing the fibres from one side to another. These act as a sequence of pivots for mechanical and functional control during contraction. Myosin filaments are equally ribbon-shaped, and in(More)
Allograft inflammatory factor-1 (AIF-1) is a 17-kDa cytokine-inducible calcium-binding protein that, in vertebrates, plays an important role in the allograft immune response. Its expression is mostly limited to the monocyte/macrophage lineage. Until recently, AIF-1 was assumed to be a novel molecule involved in inflammatory responses. To clarify this(More)
At the moment of parasitization by another insect, the host Heliothis larva is able to defend itself by the activation of humoral and cellular defenses characterized by unusual reactions of hemocytes in response to external stimuli. Here, we have combined light and electron microscopy, staining reactions, and immunocytochemical characterization to analyze(More)