Madison S. Powell

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This study describes the development of a TaqMan real-time quantitative polymerase chain reaction (QPCR) technique using the heat-shock protein 70 (Hsp 70) and 18S ribosomal DNA (18S rDNA) sequences to identify Myxobolus cerebralis and attempt to quantify infection severity within rainbow trout fry Oncorhynchus mykiss. Rainbow trout for this study were(More)
Redband trout (Oncorhynchus mykiss ssp.) in southeastern Oregon inhabit high-elevation streams that exhibit extreme variability in seasonal flow and diel water temperature. Given the strong influence and potential limitations exerted by temperature on fish physiology, we were interested in how acute temperature change and thermal history influenced the(More)
Understanding the effects of dietary carbohydrates on transcription factors that regulate myogenesis provides insight into the role of nutrient sensing by satellite cells towards myocyte differentiation. We evaluated the influence of dietary carbohydrate level (0, 15, 25 or 35%) on the temporal mRNA expression patterns (4, 8 or 12 weeks) of transcription(More)
The use of captive broodstocks is becoming more frequently employed as the number of species facing endangerment or extinction throughout the world increases. Efforts to rebuild the endangered Snake River sockeye salmon, Oncorhynchus nerka, population have been ongoing for over a decade, but the use of microsatellite data to develop inbreeding avoidance(More)
a University of Idaho, Aquaculture Research Institute, Hagerman Fish Culture Experiment Station, 3059-F National Fish Hatchery Road, Hagerman, Idaho 83332, USA b Jeju National University, Department of Marine Life Science, Jeju 690-756 and Marine and Environmental Research Institute, Jeju 695-814, South Korea c United States Department of Agriculture,(More)
In this study, we tested for the presence of sexual dimorphism in the hepatic transcriptome of the adult zebrafish and examined the effect of long term manipulation of dietary carbohydrate on gene expression in both sexes. Zebrafish were fed diets comprised of 0%, 15%, 25%, or 35% carbohydrate from the larval stage through sexual maturity, then sampled for(More)
Life history polymorphisms provide ecological and genetic diversity important to the long term persistence of species responding to stochastic environments. Oncorhynchus mykiss have complex and overlapping life history strategies that are also sympatric with hatchery populations. Passive integrated transponder (PIT) tags and parentage analysis were used to(More)
Barriers to migration are numerous in stream environments and can occur from anthropogenic activities (such as dams and culverts) or natural processes (such as log jams or dams constructed by beaver (Castor canadensis)). Identification of barriers can be difficult when obstructions are temporary or incomplete providing passage periodically. We examine the(More)
A study was conducted to test whether an anthocyanidin mixture (peonidin, cyanidin and pelargonidin chloride) modulates myogenesis in both induced and non-induced myogenic cells from juvenile rainbow trout (Oncorhynchus mykiss). We evaluated three different anthocyanidin concentrations (1×, 2.5× and 10×) at two sampling times (24 and 36h). To test for(More)
Next-generation massive parallel sequencing and bioinformatic techniques were applied to observe the differences in the transcriptome of Litopenaeus vannamei juveniles that were fed with a control diet (T1) or the same diet plus a live Ulva clathrata supplement (T2). The average size of the libraries was of 271 bp for T1 and 275 bp for T2. A total of(More)