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A protocol for in vitro propagation was developed for Viola pilosa, a plant of immense medicinal value. To start with in vitro propagation, the sterilized explants (buds) were cultured on MS basal medium supplemented with various concentrations of growth regulators. One of the medium compositions MS basal + 0.5 mg/l BA + 0.5 mg/l TDZ + 0.5 mg/l GA3 gave(More)
The current study reports on in vitro propagation and conservation of critically endangered medicinal plant Swertia chirayita. The sterilized explants (leaves) were cultured on Murashige and Skoog’s (MS) medium supplemented with 0.1 mg/L naphthalene acetic acid (NAA) and 3.0 mg/L benzyl adenine (BA) for callus induction, the same medium was used for shoot(More)
Bacterial blight caused by Xanthomonas axonopodis pv. punicae (Xap) is a major disease of pomegranate. Xap secretes effector proteins via type III secretion system (T3SS) to suppress pathogen-associated molecular pattern (PAMP)-triggered plant immunity (PTI). Previously we reported that XopN, a conserved effector of Xap, modulate in planta bacterial growth,(More)
Disease resistant cell lines of wild pomegranate were selected against bacterial blight caused by Xanthomonas axonopodis pv. punicae. Callus from cotyledon explants was exposed to purified toxic culture filtrate produced by bacteria. Initiation, maintenance of callus as well as selection process was carried out on MS medium supplemented with 2.0 mg/l BAP(More)
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