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We have examined the role that the transcription factor gut-enriched Krüppel-like factor (KLF4 or GKLF) plays in activating the enterocyte differentiation marker gene intestinal alkaline phosphatase (IAP). A yeast one-hybrid screen was used to identify proteins interacting with a previously identified cis-element (IF-III) located within the human IAP gene(More)
Intestinal alkaline phosphatase (IAP) is a small intestinal brush border enzyme that has been shown to function as a gut mucosal defense factor, but its precise mechanism of action remains unclear. We investigated the effects of IAP on specific bacteria and bacterial components to determine its molecular targets. Purulent fluid from a cecal ligation and(More)
BENCHMARKS Understanding gene regulation is of fundamental importance to virtually all biological systems. A gene is regulated through cis-acting regulatory DNA sequences (elements) in conjunction with transacting regulatory proteins. The cis-acting regulatory elements involved in transcription include, for example, the promoter, enhancer, silencer,(More)
Mice deficient in intestinal alkaline phosphatase (IAP) develop type 2 diabetes mellitus (T2DM). We hypothesized that a high level of IAP might be protective against T2DM in humans. We determined IAP levels in the stools of 202 diabetic patients and 445 healthy non-diabetic control people. We found that compared to controls, T2DM patients have approx. 50%(More)
Abbreviations: electrophoretic mobility shift assay (EMSA), gut-enriched Krüppel-like factor (KLF4, GKLF), intestinal alkaline phosphatase (IAP) * To be considered as co-first authors and having contributed equally to this work.Abstract Introduction: We have examined the role that the transcription factor, gut-enriched
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