Madhu S. Ayyagari

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Department of Chemical and Biochemical Engineering and Center for Biocatalysis and Bioprocessing, University of Iowa, Iowa City, Iowa 52242, Biotechnology Division, US. Army Natick Research, Development, & Engineering Center, Natick, Massachusetts 01 760, Department of Chemistry, University of Massachusetts at Lowell, Lowell, Massachusetts 01854, and Rohm &(More)
Activity of lipase (candida cylindracea) in reversed micelles was found to be sustained over extended periods of time in the presence of amphiphilic substrates. Esterification of palmitic or oleic acid and octanol was studied to characterize the lipase activity in AOT/isooctane reversed micelles. Complete conversion was possible even in the presence of(More)
Inhibition of the native metalloenzyme, alkaline phosphatase, in the presence of some metal ions, and the reactivation of its apoenzyme by Zn(II) ions is used to determine metal ion concentrations. Alkaline phosphatase-catalysed hydrolysis of a chemiluminescent substrate, chloro 3-(4-methoxy spiro [1,2-dioxetane-3-2'-tricyclo-[3.3.1.1]-decan]-4-yl) phenyl(More)
A novel technique for the trace analysis of metal ions Zn(II), Be(II), and Bi(III) in bulk solutions is discussed. This technique involves the generation of a chemiluminescence signal from alkaline phosphatase catalyzed hydrolysis of a phosphate derivative of 1,2-dioxetane. Zn(II) can be determined by two methods, reactivation of the alkaline phosphatase(More)
The use and application of the enzyme alkaline phosphatase in a chemiluminescence assay are discussed. The enzyme catalyzes the hydrolysis of a macrocyclic phosphate compound generating a chemiluminescence signal. On the basis of inhibition of this signal, a methodology for the detection and quantitation of organophosphorus-based pesticides has been(More)
Methodology is described for the creation of a molecular assembly consisting of the enzyme alkaline phosphatase immobilized onto a glass surface using a biotinylated conjugated copolymer, poly(3-undecylthiophene-co-3-thiophenecarboxaldehyde) 6-biotinamidohexanohydrazone. The biotinylated polymer is attached to the inside walls of a silanized glass capillary(More)
A molecular assembly in which a conjugated polymer is interfaced with a photodynamic protein is described. The conjugated polymer, functionalized with biotion, is designed such that it can be physisorbed on or chemically grown off a glass surface. The streptavidin-derivatized protein is immobilized on the biotinylated polymer matrix through the strong(More)
Enzymes catalyse several reactions that are difficult to perform with chemical catalysts and that are important in the synthesis and modification of different polymers in organic solvents. In enzyme-based synthesis, alteration of the reaction medium can have a significant influence on the molecular weight, polydispersity, yield and architecture of the(More)
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