Madhu Prasad

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Ovarian cancer is the leading cause of mortality from gynecological malignancies, often undetectable in early stages. The difficulty of detecting the disease in its early stages and the propensity of ovarian cancer cells to develop resistance to known chemotherapeutic treatments dramatically decreases the 5-year survival rate. Chemotherapy with paclitaxel(More)
INTRODUCTION The incidence of Barrett esophageal adenocarcinoma (BEAC) has been increasing at an alarming rate in western countries. In this study, we have evaluated the therapeutic potential of sulforaphane (SFN), an antioxidant derived from broccoli, in BEAC. METHODS BEAC cells were treated with SFN, alone or in combination with chemotherapeutic,(More)
Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in cruciferous vegetables such as brussels sprout and broccoli, is considered a promising chemo-preventive agent against cancer. In-vitro exposure to SFN appears to result in the induction of apoptosis and cell-cycle arrest in a variety of tumor types. However, the molecular(More)
BACKGROUND Epithelial injury occurs in many acute and chronic diseases of the colon and from simple mechanical and chemical injury in the normal colon. Because epithelial repair is so important, we developed a highly reproducible, entirely in vitro model of restitution in rat distal colon. METHODS The muscosal surface of colon mounted in Ussing chambers(More)
PURPOSE The aims of this study were to investigate telomere function in normal and Barrett's esophageal adenocarcinoma (BEAC) cells purified by laser capture microdissection and to evaluate the effect of telomerase inhibition in cancer cells in vitro and in vivo. EXPERIMENTAL DESIGN Epithelial cells were purified from surgically resected esophagi.(More)
The effect of oxidants on voltage-dependent K+ currents was examined in mouse colonic smooth muscle cells. Exposure to either chloramine-T (Ch-T), an agent known to oxidize both cysteine and methionine residues, or the colon-specific oxidant monochloramine (NH2Cl) completely suppressed the transient outward K+ current (Ito) while simultaneously enhancing(More)
It is remarkable that high ammonia concentrations can be present within the colonic lumen without compromising normal epithelial function. We investigated the impact of luminal ammonia on Cl− secretion in native tissue. Stripped human colonic mucosa and unstripped rat distal colon were used. Paired samples were mounted in modified Ussing chambers for(More)
BACKGROUND During colitis, activated neutrophils elaborate biologically active oxidants into the bowel wall. Colonic contraction, governed by plasma membrane ion channels in smooth muscle cells (SMCs), is markedly abnormal in colitis. The transient outward K(+) current (I(TO)) is an important determinant of electrical excitability in colonic SMCs. The aim(More)
We propose a new method for evaluating the adsorbed phase volume during physisorption of several gases on activated carbon specimens. We treat the adsorbed phase as another equilibrium phase which satisfies the Gibbs equation and hence assume that the law of rectilinear diameters is applicable. Since invariably the bulk gas phase densities are known along(More)
Hrnjez, Bruce J., Jaekyung C. Song, Madhu Prasad, Julio M. Mayol, and Jeffrey B. Matthews. Ammonia blockade of intestinal epithelial K1 conductance. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G521–G532, 1999.—Ammonia profoundly inhibits cAMP-dependent Cl2 secretion in model T84 human intestinal crypt epithelia. Because colonic lumen(More)
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