Madeleine Pestre-Alexandre

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Isoenzyme analysis was conducted on the tachyzoite stage of 35 Toxoplasma gondii isolates. Fifteen enzyme systems were studied after isoelectrofocusing of tachyzoite extracts in polyacrylamide or agarose gels. Six enzyme systems showed variable electrophoretic patterns: aspartate aminotransferase (EC 2.6.1.1), glutathione reductase (EC 1.6.4.2), glucose(More)
In april 1987, an epidemiologic study in Northern Togo was carried out in order to evaluate the prevalence of cysticercosis and epilepsy. The survey also aimed to investigate possible connections between these two illnesses. The study encompassed 5264 subjects over 15 years old, selected using random sampling techniques; 125 cases of cysticercosis were(More)
Toxoplasma gondii strains are usually defined by biological parameters such as pathogenicity in mice. A characterization of toxoplasma strains by biochemical techniques has not been reported. In this study, extracts of tachyzoites of 7 toxoplasma strains were compared on the basis of their isoenzyme patterns for 39 enzymes by means of isoelectrofocusing in(More)
The genetic polymorphism ofToxoplasma gondii was evaluated for 14 strains by isoenzyme and DNA analysis. The 14 strains belonged to 5 different zymodemes defined by the, variable patterns of 6 enzyme systems. A restriction-fragment-length polymorphism analysis was carried out with two endonucleases (Sal I andPst I) and two repetitive probes (TGR1E and(More)
In an attempt to obtain the continuous development of Hammondia hammondi in vitro, culture of the parasite was performed in 3 cell lines. Although organisms were present in culture fluids of feline kidney cells (CRFK) for as long as 3 mo, continuous culture was not possible. However, for the first time, cysts of H. hammondi were observed in cell culture(More)
An experiment was carried out on treatment of Trypanosoma brucei brucei experimentally infected sheep with central nervous system involvement with a 2-nitroimidazole derivative: Ro 15-0216 (Hoffmann La Roche & Co Ltd, Basel, Switzerland). After a single intramuscularly or intravenously administration (25 or 50 mg/kg) of this compound in healthy sheep,(More)
Total lipid extracts from Trypanosoma brucei brucei (T.b.b.), Trypanosoma brucei gambiense (T.b.g.) and Trypanosoma brucei rhodesiense (T.b.r.) were hydrolyzed and the liberated fatty acids were methylated before analysis by gas-liquid chromatography on capillary columns. The sums of the percentages for fatty acids from the different series were compared(More)