Maarten B J Roeffaers

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Two gram-negative insect pathogens, Xenorhabdus nematophila and Photorhabdus luminescens, produce rhabduscin, an amidoglycosyl- and vinyl-isonitrile-functionalized tyrosine derivative. Heterologous expression of the rhabduscin pathway in Escherichia coli, precursor-directed biosynthesis of rhabduscin analogs, biochemical assays, and visualization using both(More)
Metal-organic frameworks (MOFs) are a class of crystalline materials that consist of metal ions and organic ligands linked together by coordination bonds. Because of their porosity and the possibility of combining large surface areas with pore characteristics that can be tailored, these solids show great promise for a wide range of applications. Although(More)
Following its widespread use in biomedical research, fluorescence microscopy has recently been introduced in the catalysis field to study chemocatalytic processes with a high spatiotemporal resolution, a unique sensitivity down to the single molecule level and this under in situ conditions. This tutorial review is structured around the length scales that(More)
By providing a three-dimensional (3D) support to cells, hydrogels offer a more relevant in vivo tissue-like environment as compared to two-dimensional cell cultures. Hydrogels can be applied as screening platforms to investigate in 3D the role of biochemical and biophysical cues on cell behaviour using bioluminescent reporter cells. Gradients in oxygen(More)
The fast and reversible on/off switching of the fluorescence emission of the GFP-like fluorescent protein Dronpa has attracted considerable interest for applications in subdiffraction imaging. In this paper we study the use of a donut-mode beam in combination with two more overlapping laser beams to increase the imaging resolution through selective(More)
[*] Prof. B. F. Sels, G. De Cremer, E. Bartholomeeusen, Prof. D. E. De Vos Department of Microbial and Molecular Systems Katholieke Universiteit Leuven Kasteelpark Arenberg 23, B-3001 Leuven (Belgium) E-mail: Dr. T. Vosch, Dr. J.-I. Hotta, Dr. M. B. J. Roeffaers, E. Coutiño-Gonzalez, Prof. J. Hofkens Department of Chemistry,(More)
Catalytic processes on surfaces have long been studied by probing model reactions on single-crystal metal surfaces under high vacuum conditions. Yet the vast majority of industrial heterogeneous catalysis occurs at ambient or elevated pressures using complex materials with crystal faces, edges and defects differing in their catalytic activity. Clearly, if(More)
Imaging of nucleic acids is important for studying cellular processes such as cell division and apoptosis. A noninvasive label-free technique is attractive. Raman spectroscopy provides rich chemical information based on specific vibrational peaks. However, the signal from spontaneous Raman scattering is weak and long integration times are required, which(More)
Organometal halide perovskites show promising features for cost-effective application in photovoltaics. The material instability remains a major obstacle to broad application because of the poorly understood degradation pathways. Here, we apply simultaneous luminescence and electron microscopy on perovskites for the first time, allowing us to monitor in(More)
Microand mesoporous materials offer unique opportunities for catalysis thanks to their large surface area. By introducing active elements inside the pore walls of such materials, a wide range of acid–base or redox catalysts has been developed. For example, incorporation of Ti sites in silicalite resulted in the TS-1 catalyst, which is known for its high(More)