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The plasma membrane has been implicated as one of the critical targets of photodynamic action. We observed that plasma membrane depolarization is an early event in the photodynamic action of zinc phthalocyanine disulfonate in mouse myeloma cells, showing both photosensitizer concentration and light dose dependence. The depolarization was observed(More)
Curcumin [bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] was studied by means of UV-VIS absorption spectroscopy and nanosecond laser flash photolysis in 1,4-dioxane-water mixtures in a series of dioxane-water volume ratios. The transient characteristics were found to be dependent on the amount of water. In pure dioxane the triplet state of the(More)
The pharmacokinetic behaviour and phototherapeutic effectiveness of bis(di-isobutyloctadecylsil-oxy)-2,3-naphthalocyanatosilicon (iso-BOSiNc) incorporated into dipalmitoyl-phosphatidylcholine (DPPC) liposomes have been studied in Balb/c mice bearing an MS-2 fibrosarcoma. We found that iso-BOSiNc i.v.-injected at a dose of 0.5 mg kg-1 b.w. is preferentially(More)
There is widespread acceptance that singlet oxygen is a key intermediate on one of the pathways leading to the phenomenon of photodynamic action. However, the identification of this moiety within a particular biological system and the determination of a direct link between its presence and a particular photodynamic effect is a goal which photobiologists(More)
The effect of photodynamic action on plasma membranes was examined using a fluorescent potentiometric indicator [di-SBA-C2(3)] to measure alterations in the plasma membrane potential of mouse myeloma cells treated with zinc phthalocyanine sulfonate and light. Plasma membrane depolarization was observed to be an early event in photodynamic action, showing(More)
point out that hydrophobic photosensitising dyes with a porphyrin-type macrocyclic skeleton exhibit excellent tumour -localising properties both in vitro and in vivo. Such dyes, systemically injected to experimental animals, become largely associated with serum lipoproteins (Kessel, 1990); one lipoprotein class, namely low-density lipoproteins (LDL),(More)
For fabrication of effective electrochemical biosensors, interfacing the biomolecular receptor with the underlying transducer represents a critical step. The actual approach taken depends on the tethering layer covering the transducer, which is typically either a conducting polymeric matrix, or a thin film, such as an alkanethiol monolayer. Non-specific(More)
Laser flash photolysis techniques have yielded rate constants for physical and reactive quenching modes of O2(1 delta g) by nicotine, nicotinamide adenine dinucleotide (oxidized and reduced forms) and the reduced forms of nicotinamide mononucleotide, nicotinamide adenine dinucleotide phosphate and nicotinamide hypoxanthine dinucleotide. In the case of the(More)