MADURAI G. GANESAN

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The effect of various concentrations of calcium ion (Ca 2+) on dipalmitoylphosphatidyicboline (DPPC), distearoylphosphatidylcholine (DSPC) and mixed DPPC/DSPC (1:1) liposomes was studied by differential scanning calorimetry. Ca 2+ concentrations of 10 mM and above split the main transition peak of DPPC into two distinguishable components, and, at 30 mM and(More)
The interactions of calcium and neomycin with liposomes of various anionic phospholipids plus lecithin were studied by differential scanning calorimetry. Phosphatidylinositol bisphosphate differed from other acidic phospholipids in its interactions with both calcium and neomycin. Calcium, at concentrations as low as 1 mM, induced the appearance of a second(More)
The effect of various concentrations of calcium ion (Ca2+) on dipalmitoylphosphatidylcholine (DPPC), distearoylphosphatidylcholine (DSPC) and mixed DPPC/DSPC (1:1) liposomes was studied by differential scanning calorimetry. Ca2+ concentrations of 10 mM and above split the main transition peak of DPPC into two distinguishable components, and, at 30 mM and(More)
The interactions between insulin and various lipids were studied by monolayer penetration experiments at constant surface area. The increase in surface pressure, delta II, of a lipid film depended upon the particular lipid used and the concentration of insulin in the subphase. For all lipids studied, delta II was dependent on the initial surface pressure of(More)
The effect of entrapped PEG-4000, inulin, and insulin on thein situuptake from the rat jejunum of liposomes was determined. Each of these markers greatly reduced or completely eliminated jejunal uptake unless the liposomes were repeatedly “washed” to remove marker from the external bilayer surface. Addition of free marker to a suspension of empty liposomes(More)
Calcium ion (Ca2+, 5 mM) caused a large upward shift in the transition temperature (Tc) of dipalmitoyl phosphatidylglycerol liposomes, apparently interacting with external and internal lipid in the bilayer. Neomycin (1 mM) caused only a small shift, apparently not penetrating the internal lipid domains. When liposomes were first incubated with Ca2+ followed(More)
The transport of sterols incorporated into the lecithin bilayer of small unilamellar liposomes through a model membrane was studied. A two-chamber diffusion cell containing liposomes with incorporated [4-14C)cholesterol or beta-[4-14C]sitosterol in the donor chamber and liposomes with unlabeled cholesterol in the receiver chamber was used. The permeability(More)
Citrinin, a mycotoxin, was studied for its effect on artificial membranes (liposomes). Of the three (net positive, negative and neural) liposomal preparations tested, the membrane carrying a net positive charge was preferentially acted upon by citrinin and in this case even the lowest concentration was found to be sufficient for causing a high degree of(More)
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