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During oogenesis, germ cell numbers sharply decrease when meiosis is initiated. There is solid evidence (DNA ladders, in situ detection) that this loss is through apoptosis. Oocyte apoptosis appears to hit mitotic primordial germ cells (PGC), pachytene oocytes and early primordial follicles. The control of oocyte apoptosis is not fully understood, although(More)
A rapid technique for counting and classifying large ovarian follicles of domestic animals is described. Using a cryostat, 250-micrograms thick sections were cut from the frozen ovary; an image of the surface of each ovarian section was recorded on videotape. By replaying the videotape, the largest profile of each follicle larger than 1 mm in diameter was(More)
Five pony and 5 saddle-type mares were used to compare total ovarian follicular populations between breeds. The animals were hemi-ovariectomized at the preovulatory stage during the breeding season and the ovary bearing the large preovulatory follicle was studied using histological techniques. Pony and saddle-type mares did not differ as to mean number of(More)
Because twin (and sometimes triplet) births commonly occur in sheep, interactions between foetuses of opposite sex may occur during life in utero and affect adult productive or reproductive performance. This was investigated by comparing growth, milk production and reproductive features of female lambs born as twins with a sister (FF) or a brother (FM) or(More)
This paper reports the effects of X-irradiation on ovulation rate, cyclicity and progesterone and FSH levels in Ile-de-France ewes (4 control and 16 irradiated) after they were treated during the breeding season. The doses used (800 and 2 400 R) destroyed 50% of all size classes of the follicular population. Ovulation occurred in 87% of the treated ewes(More)
In the middle of the breeding season, 16 pony mares (n = 4 per day) were slaughtered on four different days (days 6, 14, 17 and the preovulatory day) of the oestrous cycle, day 0 being the day of the last ovulation. All the ovaries were examined histologically; the number, size and atresia (defined by granulosa cell pyknosis) of all follicles larger than 1(More)
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