Stat3-coordinated Lin-28–let-7–HMGA2 and miR-200–ZEB1 circuits initiate and maintain oncostatin M-driven epithelial–mesenchymal transition
It is shown that oncostatin M (OSM) is expressed in an autocrine/paracrine fashion in invasive breast carcinoma and the importance of Stat3-coordinated Lin-28B–let-7–HMGA2 and miR-200–ZEB1 circuits in the cytokine-mediated phenotypic reprogramming of breast cancer cells is highlighted.
Development of novel cell surface CD34-targeted recombinant adenoassociated virus vectors for gene therapy.
A chimeric vector combining the AAV capsid protein and the variable region of a single-chain antibody against human CD34 molecules, a cell surface marker for hematopoietic stem/progenitor cells is engineered and significantly increased the preferential infectivity of rAAV for the CD34+ human myoleukemia cell line KG-1.
A hairpin ribozyme inhibits expression of diverse strains of human immunodeficiency virus type 1.
It is shown by transient transfection that inhibition of expression of diverse strains of HIV-1 can be achieved by this ribozyme expressed in the proper vectors.
Intracellular immunization of human T cells with a hairpin ribozyme against human immunodeficiency virus type 1.
Results indicate that transfer and expression of the ribozyme gene interfere with both early and late events in the HIV replication cycle and confer long-term resistance to HIV-1 infection.
Intracellular immunization of human fetal cord blood stem/progenitor cells with a ribozyme against human immunodeficiency virus type 1.
- M. Yu, M. Leavitt, F. Wong-Staal
- Biology, MedicineProceedings of the National Academy of Sciences…
- 31 January 1995
Successful treatment of human immunodeficiency virus infection may ultimately require targeting of hematopoietic stem cells. Here we used retroviral vectors carrying the ribozyme gene to transduce…
Retroviral delivery of GAD-IgG fusion construct induces tolerance and modulates diabetes: a role for CD4+ regulatory T cells and TGF-β?
It is proposed that the induction of tolerance and the prevention of diabetes incidence in NOD female mice induced by the GAD-IgG fusion construct may require CD4+ regulatory T cells, and the possible mediation of TGF-β.
Cellular and humoral anti‐tumor immune responsiveness in chickens bearing tumors induced by avian sarcoma virus
Transformed CEF were found to produce far higher quantities of transforming virus progeny than avian RS cells, although the latter were apparently able to synthesize defective viral particles.
Efficient gene transfer to hematopoietic progenitor cells using SV40-derived vectors
We used recombinant SV40 (rSV40)-derived vectors to deliver transgenes to human and simian hematopoietic progenitor cells in culture, and in vivo after transduction ex vivo. rSV40 are highly…
Progress towards gene therapy for HIV infection.
Progress in this field is likely to be rapid and to contribute to the broader applicability of human gene therapy to the treatment of other disorders.
Transfer of an anti-HIV-1 ribozyme gene into primary human lymphocytes.
This study presents the first demonstration in primary human T cells of resistance to HIV-1 infection conferred by gene transfer, and develops a system to transfer this ribozyme gene into freshly isolated human peripheral blood lymphocytes (PBLs) using a murine retrovirus vector.