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In Vitro Synthesis of Hyaluronan by a Single Protein Derived from Mouse HAS1 Gene and Characterization of Amino Acid Residues Essential for the Activity*
The results suggest that the HAS1 protein alone is able to synthesize hyaluronan and different amino acid residues on the cytoplasmic central loop domain are involved in transferring GlcNAc and GlcA residues, respectively. Expand
Heat-inactivated proteins are rescued by the DnaK.J-GrpE set and ClpB chaperones.
Functional chaperone cooperation between Hsp70 (DnaK) and Hsp104 (ClpB) was demonstrated in vitro and it was found that TGroEL/ES could not substitute T clpB and all these components were necessary for the reactivation. Expand
Nuclear Export of Human β-Catenin Can Occur Independent of CRM1 and the Adenomatous Polyposis Coli Tumor Suppressor*
In vitro transport assays confirmed that β-catenin exits the nucleus by at least two distinct pathways, revealing a CRM1- and APC-independent pathway and implications for its regulation and its role in transferring signals between the nucleus and plasma membrane. Expand
Binding Sites and Binding Properties of Binary and Ternary Complexes of Insulin-like Growth Factor-II (IGF-II), IGF-binding Protein-3, and Acid-labile Subunit*
It was found that not only the IGFBP-3 derivatives with the amino-terminal, cysteine-rich domain (N domain) but also the synthetic peptide maintained affinity for IGF-II, and these results strongly suggest that the N domain, containing at least Glu52-Ala92, of rat IGF BP-3 is important for binding to IGF- II. Expand
Purification and Characterization of a Novel Physiological Substrate for Calcineurin in Mammalian Cells*
The ubiquitous expression of CRHSP-24 in rat tissues suggests that this novel calcineurin substrate plays a common role in calcium-mediated signal transduction. Expand
[Molecular chaperone].
Stepping rotation of F1-ATPase visualized through angle-resolved single-fluorophore imaging.
Results show that the 120 degrees stepping is a genuine property of this molecular motor and that the rate of ATP binding is insensitive to the load exerted on the rotor subunit. Expand
On the Maximum Size of Proteins to Stay and Fold in the Cavity of GroEL underneath GroES*
The maximum size of the non-native protein to stay and fold in the cis-cavity is reported, which is estimated to be up to ∼57-kDa, using total soluble proteins ofEscherichia coli in denatured state as binding substrates and protease resistance as the measure of polypeptide held in thecis-Cavity. Expand
Interaction of the Hepatitis B Virus X Protein with the Crm1-dependent Nuclear Export Pathway*
It is shown that the oncogenic hepatitis B virus (HBV) X protein (HBx) contains a functional NES motif, and it is suggested that Crm1 may play a role in HBx-mediated liver carcinogenesis. Expand
A Novel Factor Required for the Assembly of the DnaK and DnaJ Chaperones of Thermus thermophilus*
It is shown that a novel factor is necessary for the assembly of T.DnaK·DnaJ chaperone complex from Thermus thermophilus, and this finding raises a possibility that interaction between DnaK and Dna J chaperones in other organisms is also mediated by a small protein yet unnoticed. Expand