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Parental expression of the chromodomain protein Pdd1p is required for completion of programmed DNA elimination and nuclear differentiation.
Thousands of DNA elimination events occur during somatic differentiation of many ciliated protozoa. In Tetrahymena, the eliminated DNA aggregates into submacronuclear structures containing theExpand
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Isolation of micro- and macronuclei of Tetrahymena pyriformis.
Publisher Summary This chapter discusses the isolation of micro- and macronuclei of Tetrahymena Pyriformis. With the exception of a few amicronucleate or partially amicronucleate strains ofExpand
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Nongenic, bidirectional transcription precedes and may promote developmental DNA deletion in Tetrahymena thermophila.
A large number of DNA segments are excised from the chromosomes of the somatic nucleus during development of Tetrahymena thermophila. How these germline-limited sequences are recognized and excisedExpand
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Genome downsizing during ciliate development: nuclear division of labor through chromosome restructuring.
The ciliated protozoa divide the labor of germline and somatic genetic functions between two distinct nuclei. The development of the somatic (macro-) nucleus from the germinal (micro-) nucleus occursExpand
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Specific DNA rearrangements in synchronously developing nuclei of Tetrahymena.
Specific rearrangement of internal chromosomal regions occurs during development of the somatic macronucleus in Tetrahymena thermophila and results in elimination of germ-line (micronuclear) DNAExpand
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Identification of novel chromatin-associated proteins involved in programmed genome rearrangements in Tetrahymena
Extensive DNA rearrangements occur during the differentiation of the developing somatic macronuclear genome from the germ line micronuclear genome of Tetrahymena thermophila. To identify genesExpand
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Programmed DNA Deletion As an RNA-Guided System of Genome Defense
Genomewide DNA rearrangements occur in many eukaryotes during development, but their functions and mechanisms are poorly understood. Previous studies have implicated a sequence-recognition mechanismExpand
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A distant 10-bp sequence specifies the boundaries of a programmed DNA deletion in Tetrahymena.
Programmed DNA deletion occurs at thousands of specific sites in most ciliates studied. To understand the mechanism of this prominent DNA rearranging process, we analyzed one of the deletion elementsExpand
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A small number of cistrons for ribosomal RNA in the germinal nucleus of a eukaryote, Tetrahymena pyriformis.
The percentage of DNA complementary to 25S and 17S rRNA has been determined for both the macro- and micronucleus of the ciliated protozoan, Tetrahymena pyriformis. Saturation levels obtained byExpand
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Programmed DNA deletions in Tetrahymena: mechanisms and implications.
  • M. Yao
  • Biology, Medicine
  • Trends in genetics : TIG
  • 1996
Ciliated protozoa carry out remarkable DNA rearrangements during nuclear differentiation, including precise deletion of thousands of specific DNA fragments from their somatic genomes. RecentExpand
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