• Publications
  • Influence
Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1.
TLDR
Evidence is shown that LMB binds directly to CRM1 and thatCRM1 is essential for NES-dependent nuclear export of proteins in both yeast and mammalian cells, and is an essential factor for nuclear exported proteins in eukaryotes.
Mis16 and Mis18 Are Required for CENP-A Loading and Histone Deacetylation at Centromeres
TLDR
Identification of five fission yeast centromere proteins, Mis14-18, shows that Mis16 function is conserved as RbAp48 and R bAp46 are both required for localization of human CENP-A.
Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1.
TLDR
It is shown that a human kinetochore oncoprotein, AF15q14/blinkin, a member of the Spc105/Spc7/KNL-1 family, directly links spindle checkpoint proteins BubR1 and Bub1 to Kinetochores and is required for spindle checkpoints and chromosome alignment.
CRM1 is responsible for intracellular transport mediated by the nuclear export signal
TLDR
It is shown that p110 is CRM1, which is an evolutionarily conserved protein originally found as an essential nuclear protein in fission yeast and known as a likely target of LMB, which indicates that CRM 1 is an essential mediator of the NES-dependent nuclear export of proteins in eukaryotic cells.
Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1.
TLDR
Evidence is provided that such centromeric localization of hMis18 is essential for the subsequent recruitment of de novo-synthesized CENP-A, the complex of which is accumulated specifically at the telophase-G1 centromere.
The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability
TLDR
The isolation and characterization of the sad1+ gene is described and it is suggested that Sad1 is not a general component of microtubule-interacting structures per se, but is an essential mitotic component that associates with the SPB but is not required formicrotubule nucleation.
Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.
TLDR
It is reported that the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation.
Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.
TLDR
It is reported that Cut5 interacts with a novel checkpoint protein Crb2 and that this interaction is needed for normal genome maintenance and that moderate overexpression of Chk1 suppresses the phenotypes of cut5 and crb2 mutants.
A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1
TLDR
It is shown that a Mis12 core complex exists in Schizosaccharomyces pombe and human cells and that centromeric HP1 may be the base to anchor the hMis 12 core complex that is enriched with coiled coils and extends to outer Zwint-1 during mitosis.
Dynamics of centromeres during metaphase-anaphase transition in fission yeast: Dis1 is implicated in force balance in metaphase bipolar spindle.
TLDR
The Centromere showed dynamic movements throughout phase 2 as it moved back and forth and was transiently split in two before its separation, suggesting that the centromere was positioned in a bioriented manner toward the poles at metaphase.
...
1
2
3
4
5
...