Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor.
- Takayuki Tohge, Yasutaka Nishiyama, Kazuki Saito
- BiologyThe Plant Journal
- 7 March 2005
The functional genomics approach through the integration of metabolomics and transcriptomics presented here provides an innovative means of identifying novel gene functions involved in plant metabolism.
The flavonoid biosynthetic pathway in Arabidopsis: structural and genetic diversity.
- Kazuki Saito, K. Yonekura-Sakakibara, A. Fernie
- Biology, MedicinePlant physiology and biochemistry : PPB
- 1 November 2013
Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids
- R. Nakabayashi, K. Yonekura-Sakakibara, Kazuki Saito
- Environmental ScienceThe Plant Journal
- 2 December 2013
Antioxidative activity assays showed that anthocyanin overaccumulation with strong in vitro antioxidative activity mitigated the accumulation of reactive oxygen species in vivo under oxidative and drought stress, confirming the usefulness of flavonoids for enhancing both biotic and abiotic stress tolerance in crops.
Regulation of sulfur assimilation in higher plants: a sulfate transporter induced in sulfate-starved roots plays a central role in Arabidopsis thaliana.
- H. Takahashi, M. Yamazaki, K. Saito
- Environmental Science, BiologyProceedings of the National Academy of Sciences…
- 30 September 1997
The sulfate transporter (AST68) exhibited the most intensive and specific response in roots, indicating that AST68 plays a central role in the regulation of sulfate assimilation in plants.
Recent advances in the biosynthesis and accumulation of anthocyanins.
- K. Springob, J. Nakajima, M. Yamazaki, Kazuki Saito
- ChemistryNatural product reports (Print)
- 13 June 2003
This review describes biochemistry, molecular biology and regulation of anthocyanin biosynthesis, with particular emphasis on mechanistic features and late steps of anthocyanin biosynthesis including…
KNApSAcK family databases: integrated metabolite-plant species databases for multifaceted plant research.
- F. M. Afendi, Taketo Okada, S. Kanaya
- BiologyPlant and Cell Physiology
- 1 February 2012
A search engine within the KNApSAcK Core DB is developed, making it possible to search for metabolites based on an accurate mass, molecular formula, metabolite name or mass spectra in several ionization modes, and databases for retrieving metabolites related to plants used for a range of purposes are developed.
Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens.
- K. Saito, M. Kobayashi, Z. Gong, Y. Tanaka, M. Yamazaki
- BiologyThe Plant Journal
- 1999
It is shown that anthocyanidins are formed in vitro through 2-oxoglutarate-dependent oxidation of leucoanthocyanIDins catalyzed by the recombinant ANS and subsequent acid treatment.
Novel detoxification of the trichothecene mycotoxin deoxynivalenol by a soil bacterium isolated by enrichment culture
Strain E3-39 was classified as a bacterium belonging to the Agrobacterium-Rhizobium group, indicating that the 3-OH group in deoxynivalenol is likely to be involved in exerting its immunosuppressive toxicity.
Camptothecin biosynthetic genes in hairy roots of Ophiorrhiza pumila: cloning, characterization and differential expression in tissues and by stress compounds.
- Y. Yamazaki, H. Sudo, M. Yamazaki, N. Aimi, Kazuki Saito
- BiologyPlant and Cell Physiology
- 15 April 2003
The cloning and characterization of cDNAs encoding strictosidine synthase and tryptophan decarboxylase are described, two key enzymes in the biosynthesis of TIA from hairy roots of O. pumila that are presumed to be indirectly involved in camptothecin synthesis.
Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana
- K. Yonekura-Sakakibara, A. Fukushima, Kazuki Saito
- BiologyThe Plant Journal
- 14 October 2011
Two glycosyltransferases, UGT79B1 and UGT84A2, found to cluster with anthocyanin biosynthetic genes, suggest major routes from cyanid in 3-O-glucoside to the most highly modified cyanidin in the potential intricate anthokyanin modification pathways in Arabidopsis.
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