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Secretion and antigenicity of hepatitis B virus small envelope proteins lacking cysteines in the major antigenic region.
TLDR
The data raise the possibility that HBV strains carrying cysteine mutations are nonreactive in hepatitis B surface antigen-specific immunoassays, and it is demonstrated that Cys-107 is critical for the structure of the group determinant a, whereasCys-147, previously implicated in intramolecular disulfide bonding, is dispensable.
Chaperones Involved in Hepatitis B Virus Morphogenesis
TLDR
This work identifies and characterize a preS-specific sequence that confers the suppression of cotranslational translocation even of a model reporter, and proposes an essential role for calnexin, as well as for Hsc70, in chaperoning HBV assemby.
Analysis of intermolecular disulfide bonds and free sulfhydryl groups in hepatitis B surface antigen particles
TLDR
The envelope proteins of hepadnaviruses are highly cross-linked by disulfide bonds in complete virions and 20 nm subviral envelope particles and alkylation studies using N-ethyl-maleimide indicate that C76, C90, and/or C221 carry the only free sulfhydryl group(s) present in 20‬nm particles secreted from cell lines.
Role for Calnexin and N-Linked Glycosylation in the Assembly and Secretion of Hepatitis B Virus Middle Envelope Protein Particles
TLDR
It is demonstrated that a single N glycan, specific for M, is required for efficient secretion of M empty envelope particles and this glycan mediates specific association of M with the chaperone calnexin.
Sequence-specific repression of cotranslational translocation of the hepatitis B virus envelope proteins coincides with binding of heat shock protein Hsc70.
TLDR
It is demonstrated that the repression of cotranslational translocation of preS is conferred by a preS1-specific sequence and identified the cytosolic heat shock protein Hsc70 as a specific binding partner of L.
Structure‐activity relationships of acetylcholine derivatives with Lucilia cuprina nicotinic acetylcholine receptor α1 and α2 subunits in chicken β2 subunit hybrid receptors in comparison with
TLDR
The gene identification and cloning of nAChR α1 and α2 subunits (Lcα1 and Lcα2) from the sheep blowfly Lucilia cuprina are reported to provide guidance for the identification or design of insect‐specific nAchR agonists using structure‐based or in silico methods.
Molecular cloning, partial genomic structure and functional characterization of succinic semialdehyde dehydrogenase genes from the parasitic insects Lucilia cuprina and Ctenocephalides felis
TLDR
The identification, cDNA cloning and functional expression of SSADH from the parasitic insects Lucilia cuprina and Ctenocephalides felis are reported, showing that the recombinant proteins possess potent NAD+‐dependentSSADH activity, while their catalytic efficiency for other aldehyde substrates is lower.
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