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Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems
TLDR
This study reveals a protospacer DNA comprising a 23-bp duplex bracketed by tyrosine residues, together with anchored flanking 3' overhang segments, and provides important structure-based mechanistic insights into PAM-dependent spacer acquisition. Expand
Cadmium accumulation and its effects on metal uptake in maize (Zea mays L.).
TLDR
Liyu No. 6 has a greater ability to remove Cd from solution and accumulate it when compared with Nongda No. 108, and can be considered a Cd-hyperaccumulator, according to the current accepted shoot concentration. Expand
Genome-Wide Identification and Analysis of MAPK and MAPKK Gene Families in Brachypodium distachyon
TLDR
Analysis of the genomic evolution showed that both tandem and segment duplications contributed significantly to the expansion of MAPK and MAPKK families and expression studies of closely related family members among B. distachyon, Arabidopsis and rice showed that even recently duplicated representatives may fulfill different functions and be involved in different signal pathways. Expand
Construction of a human cell landscape at single-cell level
TLDR
The results provide a useful resource for the study of human biology and find that stem and progenitor cells exhibit strong transcriptomic stochasticity, whereas differentiated cells are more distinct. Expand
Genome and transcriptome analysis of the grapevine (Vitis vinifera L.) WRKY gene family
TLDR
The present study identifies a total of 59 putative grapevine WRKY transcription factors and provides a foundation for further comparative genomics and functional studies of this important class of transcriptional regulators in grapevine. Expand
Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen
TLDR
Findings suggest that the SucC/SucD-involving mechanism, instead of one based on cellulosomes or the free-enzyme system, serves a major role in lignocellulose degradation in yak rumen. Expand
Textural characteristic, antioxidant activity, sugar, organic acid, and phenolic profiles of 10 promising jujube (Ziziphus jujuba Mill.) selections.
TLDR
The results showed that ascorbic acid and proanthocyanidins contents were positively correlated with hardness, and antioxidant activity was well correlated with TP content, and Jiaxianmuzao could be an important dietary source of natural antioxidants for prevention of diseases caused by oxidative stress. Expand
Development of Glycoprotein Capture-Based Label-Free Method for the High-throughput Screening of Differential Glycoproteins in Hepatocellular Carcinoma*
TLDR
The developed method was an effective approach to quantitatively analyze glycoproteins in human serum and could be further applied in the biomarker discovery for HCC and other cancers. Expand
Calcium and Magnesium Concentration of Inbred and Hybrid Broccoli Heads
TLDR
Examining variation in Ca, and also Mg concentrations, in a collection of USDA inbreds and commercial F1 hybrids indicates that the environmental influence on phenotypic expression of Ca and Mg concentration may complicate genetic improvement of head mineral concentration. Expand
Distribution of microbial populations and their relationship with environmental parameters in the coastal waters of Qingdao, China.
TLDR
Temperature and nutrients were the synchronous factors controlling the growth of heterotrophic bacteria, pico- and nanophytoplankton in the coastal waters of Qingdao in spring, and the results suggested that synergistic and antagonistic effects existed among microbial groups. Expand
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