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The Spliceosome: Design Principles of a Dynamic RNP Machine
Ribonucleoproteins (RNPs) mediate key cellular functions such as gene expression and its regulation. Whereas most RNP enzymes are stable in composition and harbor preformed active sites, theExpand
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Structural Basis for the Function of the Ribosomal L7/12 Stalk in Factor Binding and GTPase Activation
The L7/12 stalk of the large subunit of bacterial ribosomes encompasses protein L10 and multiple copies of L7/12. We present crystal structures of Thermotoga maritima L10 in complex with three L7/12Expand
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Helical extension of the neuronal SNARE complex into the membrane
Neurotransmission relies on synaptic vesicles fusing with the membrane of nerve cells to release their neurotransmitter content into the synaptic cleft, a process requiring the assembly of severalExpand
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A NusE:NusG Complex Links Transcription and Translation
Transcription and Translation in Train In bacteria, translation of messenger RNA into proteins by the ribosome usually begins soon after the ribosome binding site emerges from RNA polymerase. NowExpand
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CASK Functions as a Mg2+-Independent Neurexin Kinase
CASK is a unique MAGUK protein that contains an N-terminal CaM-kinase domain besides the typical MAGUK domains. The CASK CaM-kinase domain is presumed to be a catalytically inactive pseudokinaseExpand
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Crystal structures of transcription factor NusG in light of its nucleic acid‐ and protein‐binding activities
Microbial transcription modulator NusG interacts with RNA polymerase and termination factor ρ, displaying striking functional homology to eukaryotic Spt5. The protein is also a translationalExpand
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Inhibition of RNA Helicase Brr2 by the C-Terminal Tail of the Spliceosomal Protein Prp8
Spliceosome Helicase Introns are removed from eukaryotic premessenger RNA by the spliceosome. The spliceosome is assembled and disassembled during the course of each splicing reaction, and theExpand
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Structural basis for reversible photoswitching in Dronpa
Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state byExpand
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Binding of the Human Prp31 Nop Domain to a Composite RNA-Protein Platform in U4 snRNP
Although highly homologous, the spliceosomal hPrp31 and the nucleolar Nop56 and Nop58 (Nop56/58) proteins recognize different ribonucleoprotein (RNP) particles. hPrp31 interacts with complexesExpand
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Determinants of Enzymatic Specificity in the Cys-Met-Metabolism PLP-Dependent Enzyme Family: Crystal Structure of Cystathionine γ-Lyase from Yeast and Intrafamiliar Structure Comparison
Abstract The crystal structure of cystathionine γ-lyase (CGL) from yeast has been solved by molecular replacement at a resolution of 2.6 å. The molecule consists of 393 amino acid residues and oneExpand
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