Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,…
Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures.
It is demonstrated that early progenitors committed to hematopoietic development could be identified by surface expression of leukosialin (CD43) and it was demonstrated that the first-appearing CD34(+)CD43(-)CD235a(+) CD41a(+/-)CD45(-) cells represent precommitted erythro-megakaryocytic progenitor.
Human embryonic stem cell-derived CD34+ cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential.
Data indicate that CD34+ cells generated through hES/OP9 coculture display several features of definitive hematopoietic stem cells.
Efficient Feeder-Free Episomal Reprogramming with Small Molecules
Improvements in episomal reprogramming efficiency are greatly improved and enabled the routine derivation of footprint-free human iPSCs from skin fibroblasts, adipose tissue-derived cells and cord blood cells.
Hematopoietic and Endothelial Differentiation of Human Induced Pluripotent Stem Cells
Although several issues remain to be resolved before iPSC‐derived blood cells can be administered to humans for therapeutic purposes, patient‐specific iPSCs can already be used for characterization of mechanisms of blood diseases and for identification of molecules that can correct affected genetic networks.
A mesoderm-derived precursor for mesenchymal stem and endothelial cells.
Identification of the hemogenic endothelial progenitor and its direct precursor in human pluripotent stem cell differentiation cultures.
Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
- Kyung-Dal Choi, M. Vodyanik, I. Slukvin
- Biology, MedicineJournal of Clinical Investigation
- 1 September 2009
A protocol for efficient generation of neutrophils, eosinophils, macrophages, osteoclasts, DCs, and Langerhans cells from human embryonic stem cells (hESCs) was developed and it was demonstrated that a similar protocol could be used to generate myelomonocytic cells from induced pluripotentstem cells (iPSCs).
Direct Induction of Hematoendothelial Programs in Human Pluripotent Stem Cells by Transcriptional Regulators
It is demonstrated that two distinct genetic programs regulate the hematopoietic development from hPSCs and that both of these programs specify h PSCs directly to hemogenic endothelial cells.
Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells
In this paper, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation…