• Publications
  • Influence
Comparative study of the stability of saxitoxin and neosaxitoxin in acidic solutions and lyophilized samples.
This paper analysed the stability of saxitoxin (STX) and neosaxitoxin in acidic solution and lyophilized samples to demonstrate that STX is very stable in solution samples and could be adopted as a reference standard. Expand
In vitro and in vivo evaluation of paralytic shellfish poisoning toxin potency and the influence of the pH of extraction.
An in vitro quantification method is developed using cultured neurons and the first data evaluating the potencies of PSP toxins after extraction in acidic pHs are provided, indicating that the toxicity of the sample increases in acidic conditions. Expand
Cytoskeletal disruption is the key factor that triggers apoptosis in okadaic acid-treated neuroblastoma cells
Results demonstrate that collapse of mitochondrial membrane potential is accessory in the execution of apoptosis, which is directly dependent on cytoskeletal changes. Expand
Azaspiracid-1, a potent, nonapoptotic new phycotoxin with several cell targets.
Results indicate that the toxin does not have an apoptotic effect but uses actin for some of its effects, and cytoskeleton seems to be an important cellular target for AZ-1 effect. Expand
Azaspiracid-4 inhibits Ca2+ entry by stored operated channels in human T lymphocytes.
First data about the effect of AZ-4 on cytosolic calcium concentration [Ca2+]i in freshly human lymphocytes are presented, showing an effect clearly different from other azaspiracid analogues. Expand
Fluorescent microplate cell assay to measure uptake and metabolism of glucose in normal human lung fibroblasts.
This first report of a fluorimetric microplate assay to assess glucose uptake and metabolism in eukaryotic cells is carried out and it is found that this cell assay is very sensitive, reproducible, provides fast results and graphical display of data. Expand
Study of cytoskeletal changes induced by okadaic acid in BE(2)-M17 cells by means of a quantitative fluorimetric microplate assay.
Results suggest direct interactions of Ser/Thr protein phosphatases with actin-binding proteins in the regulation of actin polymerization, thus indicating that disruption of cytoskeletal structure may be a key mechanism of OA-induced diarrhea. Expand
Cell growth inhibition and actin cytoskeleton disorganization induced by azaspiracid-1 structure-activity studies.
Azaspiracid-1 (AZA-1) is a marine toxin discovered 10 years ago. Since then, toxicologic studies have demonstrated that AZA-1 targets several organs in vivo, including the intestine, lymphoidExpand
The methyl ester of okadaic acid is more potent than okadaic acid in disrupting the actin cytoskeleton and metabolism of primary cultured hepatocytes
Methyl okadaate (MeOk), a methyl ester derivative of OA, was considered almost inactive due to its weak inhibition of PP1 and PP2A, but the activity and potency of MeOk in hepatic cells in comparison with that of O a and MCs are investigated. Expand
Yessotoxin Induces Er-Stress Followed By Autophagic Cell Death In Glioma Cells Mediated By mTOR and BNIP3.
It is shown that the toxin induces a deregulation of the lipid metabolism in glioma cells as a consequence of induction of endoplasmic reticulum stress, which arrests the cell cycle and inhibits the protein synthesis. Expand