Isolation of a novel arachidonic acid metabolite 3‐hydroxy‐5,8,11,14‐eicosatetraenoic acid (3‐HETE) from the yeast Dipodascopsis uninucleata UOFs‐Y128
Biotransformation of (+)limonene and (-)piperitone by yeasts and yeast-like fungi
- E. van Rensburg, N. Moleleki, J. van der Walt, P. Botes, M. V. van Dyk
- BiologyBiotechnology Letters
- 1 August 1997
Arxula adeninivorans and Yarrowia lipolytica converted (+)limonene to perillic acid and (-)pipersitone to 7-hydroxy-piperitone and two unclassified strains of the basidiomycetes transformed isopiperitenone.
Hydroxylation of ()limonene, (−)α-pinene and (−)β-pinene by a Hormonema sp.
Hormonema sp. UOFS Y-0067, freshly isolated from pine forest litter, hydroxylated mono- and bicyclic monoterpenes on the cyclohexane ring. It converted (+)limonene to trans-isopiperitenol (0.5 g/l;…
Biocatalytic resolution of 1,2-epoxyoctane using resting cells of different yeast strains with novel epoxide hydrolase activities
Good enantioselectivity (E > 200) for 1,2-epoxyoctane is reported for the first time and asymmetric hydrolysis for 8 yeast strains belonging to the genera Trichosporon, Rhodotorula, and Rhodosporidium is found.
Debaryomyces mycophilus sp. nov., a siderophore-dependent yeast isolated from woodlice.
Symbiosis among fungi, based on the availability and utilization of iron, is an aspect of mycology that has not previously been recognized and the addition of chelated iron to isolation media could lead to the discovery of many unknown yeasts and fungi.
Physico-chemical properties of the epoxide hydrolase from Rhodosporidium toruloides
- A. L. Botes, D. Litthauer, A. van Tonder, M. V. van Dyk
- Biology, ChemistryBiotechnology Letters
- 1 December 1999
It is proposed that the microsomal epoxide hydrolase from Rhodosporidium toruloides UOFS Y-0471 acts at an interface, analogous to lipases, suggesting that a Ser in the catalytic site is indispensable for substrate binding by analogy of the role of Ser residues in the related L-2-haloacid dehalogenases, as well as the ATPase and phosphatase enzymes.
Correlation between the physicochemical properties of organic solvents and their biocompatibility toward epoxide hydrolase activity in whole-cells of a yeast, Rhodotorula sp.
- J. Lotter, A. L. Botes, M. V. van Dyk, J. Breytenbach
- Biology, EngineeringBiotechnology Letters
- 1 August 2004
1-Pentanol, 2-methylcyclohexanol and 1-octanol were the most biocompatible resulting in relatively low activity losses when used at up to 20% (v/v), although the introduction of hydroxyl groups increased biOCompatibility.
Biocatalysis of nitro substituted styrene oxides by non-conventional yeasts
- C. Yeates, M. V. van Dyk, A. L. Botes, J. Breytenbach, H. Krieg
- Chemistry, BiologyBiotechnology Letters
- 1 May 2003
Yeast strains from more than 45 different genera were screened for the enantioselective hydrolysis of nitro substituted styrene oxides, finding that (R)-Styrene oxide was preferentially hydrolysed to the corresponding (R-diol with retention of configuration at the stereogenic centre.
Hydrolysis of linalyl acetate and α-terpinyl acetate by yeasts
Of 104 yeasts tested for the hydrolysis of rac-linalyl acetate and rac-α-terpinyl acetates, only one unclassified yeast isolate hydrolyzed rac-β-terpine acetate with a slight preference for the S-enantiomer (E value ∼2).
Hydrolytic kinetic resolution of the enantiomers of the structural isomers trans-1-phenylpropene oxide and (2,3-epoxypropyl)benzene by yeast epoxide hydrolase
Kinetic resolution of the enantiomers of trans-1-phenylpropene oxide and (2,3-epoxypropyl)benzene was achieved and the superiority of yeasts from the Basidiomycetes genera in theEnantioselective hydrolysis of epoxides from different structural classes is confirmed.