Gene replacement in Dictyostelium: generation of myosin null mutants.
- D. J. Manstein, M. Titus, A. De Lozanne, J. Spudich
- BiologyEMBO Journal
- 1 March 1989
The results provide genetic proof that in Dictyostelium the conventional myosin gene is required for growth in suspension, normal cell division and sporogenesis, and illustrate how gene targeting can be used as a tool in Ddictystelium.
Dictyostelium myosin I double mutants exhibit conditional defects in pinocytosis
- K. D. Novak, M. Peterson, M. Reedy, M. Titus
- BiologyJournal of Cell Biology
- 1 December 1995
It is proposed that myoA, myoB, and myoC play roles in controlling F-actin filled membrane projections that are required for pinosome internalization in suspension.
A role for myosin VII in dynamic cell adhesion
- R. Tuxworth, I. Weber, M. Titus
- BiologyCurrent Biology
- 6 March 2001
Structural basis of myosin V Rab GTPase-dependent cargo recognition
- O. Pylypenko, W. Attanda, A. Houdusse
- BiologyProceedings of the National Academy of Sciences
- 18 November 2013
This paper provides unique insights into the specific recognition of cellular compartments by the myosin V nanomotor via direct or indirect interactions with Rab GTPases and illustrates how the Rab11-binding site has been conserved during evolution and how divergence at another site of the GTD allows more specific interactions such as thespecific recognition of melanophilin by theMyosin Va isoform.
Myosin I contributes to the generation of resting cortical tension.
- J. Dai, H. Ting-Beall, R. Hochmuth, M. Sheetz, M. Titus
- BiologyBiophysical Journal
- 1 August 1999
Requirement of a Vasodilator-stimulated Phosphoprotein Family Member for Cell Adhesion, the Formation of Filopodia, and Chemotaxis in Dictyostelium* 210
- Young-hoon Han, C. Chung, R. Firtel
- BiologyJournal of Biological Chemistry
- 20 December 2002
The results provide new insights into the function of DdVASP in controlling the actin cytoskeleton during chemotaxis and filopodia formation.
SadA, a novel adhesion receptor in Dictyostelium
- P. Fey, Stephen Stephens, M. Titus, R. Chisholm
- BiologyJournal of Cell Biology
- 23 December 2002
The data combined suggest that sadA is the first substrate adhesion receptor to be identified in Dictyostelium and thatletion of the sadA gene by homologous recombination recreated the original mutant phenotype.
A class VII unconventional myosin is required for phagocytosis
- M. Titus
- BiologyCurrent Biology
- 18 November 1999
Multiple actin-based motor genes in Dictyostelium.
- M. Titus, H. Warrick, J. Spudich
- BiologyCell Regulation
- 1 November 1989
The Dictyostelium genome was probed at low stringency with a gene fragment containing the conserved conventional myosin head domain sequences to identify other actin-based motors that may play a role in the observed motility of these mutant cells.
Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes
- O. Pylypenko, Tobias Welz, E. Kerkhoff
- BiologyeLife
- 13 September 2016
How Spir actin nucleators and myosin V motors coordinate their specific membrane recruitment is reported, showing how synchronized nucleator and motor targeting could provide a common mechanism to control force generation and motility in different cellular processes.
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