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Immobilization and stabilization of recombinant multimeric uridine and purine nucleoside phosphorylases from Bacillus subtilis.
The derivatives prepared were successfully used in the synthesis of 2'-deoxyguanosine by enzymatic transglycosylation in aqueous solution between 2'- deoxyuridine and guanine. Expand
The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases.
Biochemical studies of wild-type SpUP showed that its substrate specificity is similar to that of EcUP, while EcUP is ∼7-fold more efficient than SpUP, suggesting that the negative charge in the transition state resides mostly on uracil O2, in contrast to EcUP for which transition state stabilization occurs mostly at O4. Expand
Immobilized penicillin G acylase as reactor and chiral selector in liquid chromatography.
The HPLC columns based on immobilized PGA combine the hydrolytic activity and the chiral recognition properties of PGA, therefore they have been used for the development of a combined reaction-separation system for chiral and achiral substrates. Expand
Different phyllosilicates as supports for lipase immobilisation
The aim of this work was to determine the enzymatic activities resulting from the adsorption of Rhizomucor miehei lipase (RML) and Candida cylindracea lipase (CCL) onto three differentExpand
Improvement of Catalytic Properties of Escherichia coli Penicillin G Acylase Immobilized on Glyoxyl Agarose by Addition of a Six-Amino-Acid Tag
This modification improved the immobilization efficiency of PGA on glyoxyl agarose and the catalytic properties of the PGA derivative, although it impaired the posttranslational steps of overexpressed protein maturation. Expand
Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase.
The catalytic behavior of the different PFL derivatives in the hydrolytic resolution of fully soluble (R,S) 2-hydroxy 4-phenyl butanoic acid ethyl ester in aqueous medium was analyzed and the enantioselectivity of the PFL-immobilized enzyme was significantly improved. Expand
Use of aqueous two-phase systems for in situ extraction of water soluble antibiotics during their synthesis by enzymes immobilized on porous supports.
Yields of kinetically controlled synthesis of antibiotics catalyzed by penicillin G acylase from Escherichia coli (PGA) have been greatly increased by continuous extraction of water soluble productsExpand
Influence of the enzyme derivative preparation and substrate structure on the enantioselectivity of penicillin G acylase
Abstract The immobilization of an enzyme on solid support can give a catalyst with improved stability and catalytic properties compared to the native enzyme; in this work the enantioselectivity ofExpand
Synthesis of 2′‐Deoxynucleosides by Transglycosylation with New Immobilized and Stabilized Uridine Phosphorylase and Purine Nucleoside Phosphorylase
Multimeric uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) of Bacillus subtilis have been expressed from genes cloned in Escherichia coli, purified, characterized, immobilizedExpand
Evaluation of different enzymes as catalysts for the production of β-lactam antibiotics following a kinetically controlled strategy
Abstract Several β-lactam acylases produced by different microorganisms ( Escherichia coli , Kluyvera citrophila , Acetobacter turbidans , and Bacillus megaterium ) have been evaluated as catalystsExpand