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A simple and rapid method for generating a deletion by PCR.
TLDR
A simple and rapid mutational method by using polymerase chain reaction (PCR) in which oligonucleotide primers are designed in inverted tail-to-tail directions to amplify the cloning vector together with the target sequence, which shows excellent efficiency of 100% with only one mjsincorporation. Expand
New delivery system for plasmid DNA in vivo using atelocollagen as a carrier material: the Minipellet
New delivery system for plasmid DNA in vivo using atelocollagen as a carrier material: the Minipellet
Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo.
TLDR
Atelocollagen complexed with siRNA is resistant to nucleases and is efficiently transduced into cells, thereby allowing long-term gene silencing and showing efficient inhibition of tumor growth in an orthotopic xenograft model of a human non-seminomatous germ cell tumor. Expand
Growth regulation of human prostate cancer cells by bone morphogenetic protein-2.
TLDR
Semiquantitative RT-PCR showed thatBMPR-IB mRNA was induced by androgen in the androgen-sensitive human prostatic cancer cell line LNCaP, whereas the expression of BMPR-IA and BM PR-II mRNAs was not affected by androgens, and suggested that BMPRs and their receptors differentially modulate prostate cancer cell growth in response to BMP under different hormonal conditions. Expand
Frequent association of p53 gene mutation in invasive bladder cancer.
TLDR
Structural alterations of the p53 gene were investigated to elucidate the molecular biological difference between superficial and invasive bladder cancer by polymerase chain reaction single-strand conformation polymorphism analysis and indicated that the incidence of p 53 gene mutations appeared to be much higher in invasive-type and high-grade bladder cancers than in superficial and low-grade ones. Expand
Markedly increased amounts of messenger RNAs for vascular endothelial growth factor and placenta growth factor in renal cell carcinoma associated with angiogenesis.
TLDR
Results suggest that VEGF, PIGF, and basic fibroblast growth factor are cooperatively working to increase the angiogenesis in renal cell carcinoma in vivo. Expand
Preferential alternative splicing in cancer generates a K-sam messenger RNA with higher transforming activity.
TLDR
The variant K-sam complementary DNA lacks tyrosine 769, which is a putative phospholipase C gamma 1 association site, and showed a higher transforming activity to NIH3T3 cells than the untruncated form, which was identical with the keratinocyte growth factor receptor. Expand
AMPLIFICATION OF c-erbB-2 ONCOGENE IN HUMAN ADENOCARCINOMAS IN VIVO
There are two genes related to the viral erbB gene in the human genome. c-erbB-1 is the same as the gene for the epithelial-growth-factor (EGF) receptor, and c-erbB-2 encodes a receptor-like proteinExpand
K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes.
DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequenceExpand
Cloning, expression and chromosomal localization of Wnt-13, a novel member of the Wnt gene family.
TLDR
The results suggest that the Wnt-13 gene may be involved in normal human development or differentiation as well as in human carcinogenesis. Expand
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