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A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging
Signals in many biological processes can be amplified by recruiting multiple copies of regulatory proteins to a site of action. Harnessing this principle, we have developed a protein scaffold, aExpand
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Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes
How dynein makes the right moves The molecular motor cytoplasmic dynein moves a wide range of different intracellular cargoes. Dynein's activity in vivo requires another protein, dynactin, butExpand
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Kif15 Cooperates with Eg5 to Promote Bipolar Spindle Assembly
BACKGROUND The formation of a bipolar spindle is an essential step during cell division. Bipolar spindle assembly is driven by the highly conserved microtubule motor Eg5 (kinesin-5), which can slideExpand
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Bicaudal D2, Dynein, and Kinesin-1 Associate with Nuclear Pore Complexes and Regulate Centrosome and Nuclear Positioning during Mitotic Entry
Mammalian Bicaudal D2 is the missing molecular link between cytoplasmic motor proteins and the nucleus during nuclear positioning prior to the onset of mitosis.
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A Complex of Kif18b and MCAK Promotes Microtubule Depolymerization and Is Negatively Regulated by Aurora Kinases
INTRODUCTION Spindle assembly requires tight control of microtubule (MT) dynamics. This is dependent on a variety of MT binding proteins and their upstream regulators. The Aurora kinases have severalExpand
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Systematic dissection of dynein regulators in mitosis
A comprehensive survey of the roles of dynein subunits and adaptors in mitosis reveals that dynein forms distinct complexes requiring specific recruiters and activators to promote orderly progressionExpand
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Wee1 controls genomic stability during replication by regulating the Mus81-Eme1 endonuclease
Wee1 is essential for normal DNA replication and for genomic stability, at least in part by inhibiting a general DNA damage response induced by the Mus81-Eme1 endonuclease.
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RAMA1 is a novel kinetochore protein involved in kinetochore-microtubule attachment
During mitosis, kinetochores need to attach to microtubules emanating from spindle poles. Several protein complexes have been shown to mediate the kinetochore-microtubule interaction. However, withExpand
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Dynein, Lis1 and CLIP‐170 counteract Eg5‐dependent centrosome separation during bipolar spindle assembly
Bipolar spindle assembly critically depends on the microtubule plus‐end‐directed motor Eg5 that binds antiparallel microtubules and slides them in opposite directions. As such, Eg5 can produce theExpand
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Regulation of mRNA translation during mitosis
Passage through mitosis is driven by precisely-timed changes in transcriptional regulation and protein degradation. However, the importance of translational regulation during mitosis remains poorlyExpand
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