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Rapid identification of flavivirus using the polymerase chain reaction.
  • M. Tanaka
  • Biology, Medicine
  • Journal of virological methods
  • 1 March 1993
A rapid and accurate detection and identification system was developed for flaviviruses that makes use of reverse transcription-polymerase chain reaction (RT-PCR). A primer pair (YF-1 and YF-3),Expand
Rapid identification of dengue virus serotypes by using polymerase chain reaction.
Four primer pairs were selected on the basis of the published sequence data of four dengue virus serotypes so that each unique target sequence size could be amplified for each serotype by polymeraseExpand
S-15183a and b, new sphingosine kinase inhibitors, produced by a fungus.
In the course of our screening for inhibitors of sphingosine kinase, we found two active compounds in a culture broth of a fungus, Zopfiella inermis SANK 15183. The structures of the compounds, namedExpand
Rapid detection of virus genome from imported dengue fever and dengue hemorrhagie fever patients by direct polymerase chain reaction
Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT‐PCR) without any RNAExpand
Applications of Polymerase Chain Reaction for Identification of Dengue Viruses Isolated from Patient Sera
A simple and sensitive procedure of reverse transcriptase polymerase chain reaction (RT‐PCR) was developed previously such that all 4 serotypes of dengue viruses could be detected and their serotypesExpand
Isolation of porcine adenovirus from the respiratory tract of pigs in Japan.
Japanese Encephalitis Virus Fusion Protein with Protein A Expressed in Escherichia coli Confers Protective Immunity in Mice
A complementary DNA (cDNA) that codes C‐terminal, one‐third of envelope glycoprotein (E) and N‐terminal 65 amino acids of NS1 protein of Japanese encephalitis (JE) virus was inserted into EscherichiaExpand
Characterization of a parvovirus isolated from the diarrheic feces of a pig.
A small DNA virus was isolated from the feces of a sow with diarrhea and identified as a parvovirus on the basis of its properties. The virus replicated preferentially in cell cultures of swineExpand
Infrasternal mediastinoscopic surgery for anterior mediastinal masses
BackgroundInfrasternal mediastinoscopic surgery is a new alternative to the thoracoscopic approach for patients with anterior mediastinal masses.MethodsWe applied this technique to 18 thymectomies,Expand
Establishment of the host-vector system for Micromonospora griseorubida.
Micromonosporastrains produce some important antibiotics, such as gentamicins1*, sisomicins2), sagamicins3), fortimicin A4) and mycinamicins5). We discovered mycinamicins, 16-membered macrolideExpand
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