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Transglutaminase-mediated N- and C-terminal fluorescein labeling of a protein can support the native activity of the modified protein.
TLDR
The possibility of fluorescein labeling via chemical labeling and via TGase-catalyzed modification was compared and the latter method was found to be very practical and overcame some of the problems associated with the specificity of the former. Expand
Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs.
TLDR
Intramolecular photoinduced electron transfer (ET) was observed as the decrease of intensity in steady-state fluorescence spectroscopy and as the shortening of fluorescence decaytimes and quenching data indicated that the ET rate reflects the detailed structure of the protein. Expand
Fission yeast Ubr1 ubiquitin ligase influences the oxidative stress response via degradation of active Pap1 bZIP transcription factor in the nucleus
TLDR
It is reported here an additional regulatory mechanism that Ubr1 ubiquitin ligase‐dependent degradation lowered the Pap1 protein levels, indicating that either nuclear export or Ubr2‐mediated proteolysis must be operative to prevent uncontrolled Pap1 function. Expand
A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin.
TLDR
A novel highly fluorescent nonnatural amino acid (beta-(N-methylanthraniloyl)-L-alpha,beta-diaminopropionic acid; nmaDap) was incorporated at the 120th position of streptavidin by a CGGG/CCCG four-base codon/anticodon pair. Expand
A non‐natural amino acid for efficient incorporation into proteins as a sensitive fluorescent probe
TLDR
A small and highly fluorescent non‐natural amino acid that contains an anthraniloyl group (atnDap) was incorporated into various positions of streptavidin and showed unique properties that indicate that it is the most suitable non‐ natural amino acid for a position‐specific fluorescent labeling of proteins that is highly sensitive to microenvironmental changes. Expand
Synthesis of a novel histidine analogue and its efficient incorporation into a protein in vivo.
TLDR
Results suggest that the hydrogen atom at a specific position seriously affects incorporation in histidine analogues, and suggest that proteins containing unnatural amino acids have immense potential in biotechnology and medicine. Expand
Position-specific incorporation of a highly photodurable and blue-laser excitable fluorescent amino acid into proteins for fluorescence sensing.
TLDR
The high incorporation efficiency, the high photodurability, the excitability with blue-lasers, and high sensitivity to the environment make the acridonylalanine as the promising fluorescent amino acid for sensing small molecules when incorporated into specific positions of various antibodies, receptors, and enzymes. Expand
Construction of a crown ether-like supramolecular library by conjugation of genetically-encoded peptide linkers displayed on bacteriophage T7.
TLDR
By using the 10BASEd-T, a crown ether-like macrocyclic library possessing randomized peptide linkers on bacteriophage T7 is synthesized and a specific binder for the N-terminal domain of Hsp90 is obtained. Expand
Selective Functionalization on [60]Fullerene Governed by Tether Length
In order to accomplish the selective synthesis of [60]fullerene bisadducts, the reactions of [60]fullerene with compounds in which two α,α‘-dibromo-o-xylene moieties were connected by anExpand
Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
TLDR
In the M13 system, Pro or a basic amino acid should be avoided at the N-terminus of peptide fused to gp3, and peptides containing odd number(s) of Cys should be designed with caution in the T7Select system. Expand
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