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An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190.
The results indicate that orfD encodes an unusual IPP isomerase showing no sequence similarity to those of IPp isomerases identified to date, considered to be a suitable molecular target for the screening of antibacterial drugs specific to S. aureus.
Contribution of the Mevalonate and Methylerythritol Phosphate Pathways to the Biosynthesis of Gibberellins inArabidopsis *
Gas chromatography-mass spectrometry analyses demonstrated that both MVA and MEP pathways can contribute to the biosyntheses of GAs and campesterol, a cytosolic sterol, in Arabidopsisseedlings and provided evidence to suggest that the MVA pathway still contributes to GA biosynthesis when this pathway is limiting.
Bacillus thermoglucosidasius sp. nov., a New Species of Obligately Thermophilic Bacilli.
A group of 6 strains of obligately thermophilic spore-formers is described as a novel species named Bacillus thermoglucosidasius, which produce terminally swollen sporangia and exo-oligo-1,6-glucosidase in large amounts.
Isolation of azaspiracid-2 from a marine sponge Echinoclathria sp. as a potent cytotoxin.
A combination of HPLC using ODS, GS320, and Phenylhexyl stationary phases permitted the purification without using acid or inorganic additives in the mobile phase of Azaspiracid-2 to exhibit potent cytotoxicity against P388 cells.
Cloning and Characterization of 1-Deoxy-d-Xylulose 5-Phosphate Synthase fromStreptomyces sp. Strain CL190, Which Uses both the Mevalonate and Nonmevalonate Pathways for Isopentenyl Diphosphate
The recombinant enzyme overexpressed in Escherichia coli was purified as a soluble protein and characterized, showing the same enzymatic properties of E. coli DXP synthases, although these two enzymes had different origins.
Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp.
Three Gram-positive, NaCl-requiring actinobacteria were isolated from a marine sponge, Haliclona sp., collected from the coast of Tateyama City, Japan. Comparison of 16S rRNA gene sequences indicated
Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites.
Production of metabolites in some transformants of the versatile host was higher than that of the original producers, and cryptic biosynthetic gene clusters in the original producer were also expressed in a versatile host.
Streptomyces associated with a marine sponge Haliclona sp.; biosynthetic genes for secondary metabolites and products.
This is the first comprehensive study detailing the isolation of actinobacteria from a marine sponge and novel secondary metabolites from these strains, and JBIR-34 and -35 were particularly interesting because of their unique chemical skeleton.
Formation of 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol from 2-C-methyl-d-erythritol 4-phosphate by 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate
2-C-Methyl-d-erythritol 4-phosphate is transformed to 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol in the presence of cytidine 5′-triphosphate by a novel Escherichia coli enzyme,
Characterization of 1-Deoxy-d-xylulose 5-Phosphate Reductoisomerase, an Enzyme Involved in Isopentenyl Diphosphate Biosynthesis, and Identification of Its Catalytic Amino Acid Residues*
Results indicate that Glu231 of E. coliDXP reductoisomerase plays an important role(s) in the conversion of DXP to 2-C-methyl-d-erythritol 4-phosphate, and that His153, His209, and His257, in part, associate with DXP binding in the enzyme molecule.