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L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein
TLDR
Analyzing an avirulent Tn917-lac mutant defective for actin polymerization showed that the actA gene encodes a surface protein necessary for bacterially induced actin assembly, and results indicate that it is a 610 amino acid protein with an apparent molecular weight of 90 kd. Expand
Optimization of the cutoff value for the Aspergillus double-sandwich enzyme immunoassay.
TLDR
A cutoff OD index of 0.5--identical to the approved cutoff in the United States--improves the overall performance of the PA-EIA for adult hematology patients. Expand
New Enzyme Immunoassays for Sensitive Detection of CirculatingCandida albicans Mannan and Antimannan Antibodies: Useful Combined Test for Diagnosis of Systemic Candidiasis
TLDR
Observations suggest that use of both enzyme immunoassays may be useful for the routine diagnosis of candidiasis, which clearly demonstrate a disparity between circulation of a given mannan catabolite and antimannan antibody response. Expand
Detection of galactomannan antigenemia by enzyme immunoassay for the diagnosis of invasive aspergillosis: variables that affect performance.
TLDR
Results demonstrated that decreasing the index cutoff for positivity to 0.5 increased the GM EIA's sensitivity, with minimal loss of specificity, and the low cutoff increased the duration of test positivity before diagnosis by clinical means. Expand
Galactomannoproteins of Aspergillus fumigatus
TLDR
The characterization of two glycoproteins of A. fumigatus with an N-glycan containing galactofuranose is reported, which indicates that it is a mixture of Hex5-13HexNAc2 oligosaccharides, the major molecular species corresponding to Hex6-8Hex NAc2. Expand
Recombinant antigens as diagnostic markers for aspergillosis.
TLDR
Results suggest that recombinant RNU, CAT, and DPPV have a great potential in the serodiagnosis of all forms of aspergillosis in the immunocompromised and Immunocompetent patient. Expand
Combined detection of mannanaemia and antimannan antibodies as a strategy for the diagnosis of systemic infection caused by pathogenic Candida species.
TLDR
The results suggest that regular serological monitoring for both mannanaemia and anti-mannan antibodies in at-risk patients may contribute to the early diagnosis of candidosis. Expand
Comparison of an enzyme immunoassay and latex agglutination test for detection of galactomannan in the diagnosis of invasive aspergillosis
TLDR
The results favour the use of EIA for early diagnosis and monitoring of aspergillosis in BMT patients, although the predictive value of transient positivity remains to be ascertained. Expand
Biotin sulfone as a new tool for synthetic oligosaccharide immobilization: application to multiple analysis profiling and surface plasmonic analysis of anti-Candida albicans antibody reactivity
TLDR
The application of this new strategy for the analysis of anti- Candida albicans antibody response through multiple-analyte profiling technology (Luminex) and with surface plasmonic analysis using biotin tagged synthetic oligosaccharides on avidin coated surfaces was validated using monoclonal antibodies. Expand
Analysis of surface proteins of Listeria in relation to species, serovar and pathogenicity.
TLDR
Results confirmed, for the first time, the classification of Listeria obtained previously by genomic studies and identified major, surface-located protein antigens, specific for L. monocytogenes, which should help in the identification of new virulence factors and the development of easier and more specific methods of detection and identification. Expand
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