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Antimicrobial Activity of a Bovine Hemoglobin Fragment in the Tick Boophilus microplus *
The antibacterial activity detected in tick gut contents is the result of enzymatic processing of a host protein, hemoglobin, which may be used by ticks as a defense against microorganisms. Expand
Oxidative damage to ferritin by 5-aminolevulinic acid.
Findings that ALA overload causes iron mobilization in brain and liver of rats may help explain organ-specific toxicities and carcinogenicity of ALA in experimental animals and patients with porphyria. Expand
Implementing stepwise solvent elution in sequential injection chromatography for fluorimetric determination of intracellular free amino acids in the microalgae Tetraselmis gracilis.
The concept of sequential injection chromatography (SIC) was exploited to automate the fluorimetric determination of amino acids after pre-column derivatization with o-phthaldialdehyde (OPA) in presence of 2-mercaptoethanol (2MCE) using a reverse phase monolithic C(18) stationary phase to achieve intracellular free amino acids in the green alga Tetraselmis gracilis. Expand
Fluorimetric determination of intra- and extracellular free amino acids in the microalgae Tetraselmis gracilis (Prasinophyceae) using monolithic column in reversed phase mode.
The monolithic phase facilitates the analysis by shortening the separation time and saving solvents and instrumentation costs (indeed conventional HPLC instrumentation can be used, running at lower pressures than those ones used with packed particle columns). Expand
Sulphur γ-glutamyl peptides in mature seeds of common beans (Phaseolus vulgaris L.)
Abstract The non-protein fractions of five common bean varieties ( Phaseolus vulgaris L. ) have been studied in order to determine their total contents of γ -glutamyl-S-methyl-L-cysteine ( γExpand
Biological and structural characterization of new linear gomesin analogues with improved therapeutic indices.
Biological and structural features of new linear Gm analogues, obtained by combining the removal of both disulfide bridges and the incorporation of a D- or L-proline are reported, demonstrating that designing cysteine-free analogues can improve the therapeutic index of Gm derivatives. Expand
Solid-phase peptide synthesis at elevated temperatures: a search for and optimized synthesis condition of unsulfated cholecystokinin-12.
Under the optimized synthesis condition found in these studies (temperature of 60 degrees C, DIC/HOBt as coupling reagent and 25% DMSO/toluene as solvent), no difficulties related to the aggregation phenomenon were encountered. Expand
Synthesis and in vitro evaluation of potential antichagasic hydroxymethylnitrofurazone (NFOH-121): a new nitrofurazone prodrug.
The mutagenicity tests showed that the prodrug was less toxic than the parent drug, and the synthesis of mutual prodrugs of nitrofurazone with primaquine, using specific and nonspecific spacer groups, was successful. Expand
Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptides
It is postulate that hemocidins may be important for the control of tick pathogens and midgut flora and their role in immunity is still poorly understood. Expand
Truncation of amidated fragment 33-61 of bovine alpha-hemoglobin: effects on the structure and anticandidal activity.
It is reported that Hb33-61a is lethal to Candida albicans at 6.25 microM probably through disruption of its plasma membrane and it is hypothesized that these two structured regions, by fluctuating independently in the lipid environment, may act in a coordinated fashion disrupting the yeast plasma membrane. Expand