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Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis.
The alpha-ketoglutarate dioxygenase, thymine 7-hydroxylase (EC 1.14.11.6), has been purified from cultures of Rhodotorula glutinis grown with thymine as a nitrogen source. The purification schemeExpand
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CONVERSION OF THYMINE TO 5-HYDROXYMETHYLURACIL IN A CELL-FREE SYSTEM.
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Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase.
Abstract A purification scheme has been developed for an enzyme, thymine 7-hydroxylase, which appears to catalyze three sequential dioxygenase reactions, i.e., thymine → 5-hydroxymethyluracil →Expand
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Studies pertaining to the purification and properties of thymine 7-hydroxylase.
Abstract 1. 1. It has been shown that the thymine 7-hydroxylase activity of cell-free extracts prepared from cultures of Neurospora crassa, grown under forced aeration in liquid media, is usuallyExpand
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3 Oxygenases: Dioxygenases
Publisher Summary Pyrocatechase was isolated from cells of a pseudomonad, which catalyzed oxidative ring cleavage of the benzene ring of catechol forming cis, cis-muconic acid as the reactionExpand
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Thymine 7-hydroxylase and pyrimidine deoxyribonucleoside 2' -hydroxylase activities in Rhodotorula glutinis.
Cell-free preparations from Rhodotorula glutinis catalyzed the conversion of deoxyribonucleosides to ribonucleosides in a pyrimidine deoxyribonucleoside 2' -hydroxylase reaction. The reactionExpand
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Metabolism of pyrimidine deoxyribonucleosides in Neurospora crassa.
The experiments in this report involve the following series of reactions which were previously demonstrated with purified enzyme preparations from Neurospora crassa: thymidine a yields thymineExpand
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Regulation of thymidine metabolism in Neurospora crassa.
The utilization of thymidine by Neurospora crassa is initiated by the pyrimidine deoxyribonucleoside 2'-hydroxylase reaction and the consequent formation of thymine and ribose. Thymine must then beExpand
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Substitution of nucleoside triphosphates for ascorbate in the thymine 7-hydroxylase reaction of Rhodotorula glutinis.
Nucleoside di- and triphosphates substituted for ascorbate in the thymine 7-hydroxylase reaction in studies carried out with purified preparations from Rhodotorula glutinis. The stimulations broughtExpand
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