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Irrigation water as source of foodborne pathogens on fruit and vegetables.
Groundwater, surface water, and human wastewater are commonly used for irrigation and bacteria and protozoa tend to show the poorest survival outside a human host, whereas viruses and helminths can remain infective for months to years.
Genome evolution in major Escherichia coli O157:H7 lineages
CGH data suggest the existence of two dominant lineages as well as LSPA type and PT-related subgroups within E. coli O157:H7, which supports the phylogeny that has been inferred from other methods and further suggests that genomic divergence from an ancestral form and lateral gene transfer have contributed to their evolution.
Survey of Ontario Bulk Tank Raw Milk for Food-Borne Pathogens.
The results confirm the presence of bacterial food pathogens in raw milk and emphasize the importance of continued diligence in the application of hygiene programs within dairies and the separation of raw milk from pasteurized milk and milk products.
Genotypic Characterization and Prevalence of Virulence Factors among Canadian Escherichia coli O157:H7 Strains
The LSPA6 genotype- and PT-related characteristics identified may be important markers of specific ecotypes of E. coli O157:H7 that have unique epidemiological and virulence characteristics.
Microbial assessment of irrigation water used for production of fruit and vegetables in Ontario, Canada.
With the exception of fecal streptococci, which increased in number toward the end of the study, none of the indicators displayed a significant trend over the course of the season, as determined by linear regression analysis of indicator concentrations over time.
Rapid Determination of Escherichia coli O157:H7 Lineage Types and Molecular Subtypes by Using Comparative Genomic Fingerprinting
CGF had equal resolution to PFGE typing but with greater specificity, generating fingerprints that were unique among phenotypically related E. coli O157:H7 lineages and PT.
Analysis of whole-cell fatty acid profiles of verotoxigenic Escherichia coli and Salmonella enteritidis with the microbial identification system
- M. Steele, W. Mcnab, J. Odumeru
- Medicine, BiologyApplied and environmental microbiology
- 1 February 1997
Differentiation of strains within bacterial species, based on gas chromatographic analysis of whole-cell fatty acid profiles, was assessed with 115 strains of verotoxigenic Escherichia coli and 315…
Evaluation of universal preenrichment broth for the recovery of foodborne pathogens from milk and cheese.
- J. Jiang, C. Larkin, M. Steele, C. Poppe, J. Odumeru
- Biology, MedicineJournal of dairy science
- 1 November 1998
Universal preenrichment broth performed better than Listeria enrichment broth in supporting the recovery and growth of heat-injured L. monocytogenes and equally as well as buffered peptone water or trypticase soy broth in support the growth of unin injured L. coli.
Identification of Escherichia coli O157:H7 Genomic Regions Conserved in Strains with a Genotype Associated with Human Infection
High-resolution genotyping shows that the O157 populations have diverged into two different lineages that appear to have different ecologies, and several potential virulence factors were identified within these regions, including a putative hemolysin-activating protein, an iron transport system, andSeveral possible regulatory genes.
Evaluation of Accuracy and Repeatability of Identification of Food-Borne Pathogens by Automated Bacterial Identification Systems
Five automated microbial identification systems were assessed for their ability to accurately and repeatedly identify six common food-borne pathogens and all systems had >80% sensitivities for the identification of Salmonella species and Listeria species at the genus level.