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Systems survey of endocytosis by multiparametric image analysis
TLDR
A new strategy to phenotypically profile the human genome with respect to transferrin (TF) and epidermal growth factor (EGF) endocytosis is developed by combining RNA interference, automated high-resolution confocal microscopy, quantitative multiparametric image analysis and high-performance computing.
IC261, a specific inhibitor of the protein kinases casein kinase 1-delta and -epsilon, triggers the mitotic checkpoint and induces p53-dependent postmitotic effects
TLDR
Evidence is provided that 3-[(2,4,6-trimethoxyphenyl)methylidenyl]-indolin-2-one (IC261), a novel inhibitor of CK1δ and CK1ε, triggers the mitotic checkpoint control and Immunfluorescence images show that at low concentrations IC261 leads to centrosome amplification causing multipolar mitosis.
Image-based analysis of lipid nanoparticle–mediated siRNA delivery, intracellular trafficking and endosomal escape
TLDR
It is estimated that escape of siRNAs from endosomes into the cytosol occurs at low efficiency (1–2%) and only during a limited window of time when the LNPs reside in a specific compartment sharing early and late endosomal characteristics.
The Role of the Casein Kinase 1 (CK1) Family in Different Signaling Pathways Linked to Cancer Development
TLDR
The functions of CK 1 are summarized and the participation of CK1 in signal transduction pathways linked to cancer development is outlined.
Mammalian CORVET Is Required for Fusion and Conversion of Distinct Early Endosome Subpopulations
TLDR
Results demonstrate that CORVET plays distinct roles at multiple stages in the mammalian endocytic pathway and suggest that CORvET plays a role in endosome fusion independently of EEA1.
Inhibition of casein kinase I delta alters mitotic spindle formation and induces apoptosis in trophoblast cells
TLDR
It is indicated that CKIδ plays an important role in the mitotic progression and in the survival of cells of trophoblast origin and IC261 could provide a new tool in treating choriocarcinomas.
Interaction of casein kinase 1 delta (CK1delta) with post-Golgi structures, microtubules and the spindle apparatus.
TLDR
In interphase cells of various mammalian species casein kinase 1 delta (CK1delta) specifically interacts with the trans Golgi network and cytoplasmic, granular particles that associate with microtubules, and its ability to phosphorylate several microtubule-associated proteins points to a regulatory role of CK1d delta at mitosis.
CellProfiler and KNIME: open source tools for high content screening.
TLDR
The Open Source software CellProfiler for image analysis and KNIME for data analysis and data mining that provide software solutions which increase flexibility and keep costs low are presented and discussed.
Identification of siRNA delivery enhancers by a chemical library screen
TLDR
This study suggests that a pharmacological approach can improve the delivery of siRNAs in a system-specific fashion, by exploiting distinct mechanisms and acting upon multiple cell types.
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