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Evidence for the presence of a secondary structure at the dibasic processing site of prohormone: the pro‐ocytocin model.
Bioactivation of pro‐proteins by limited proteolysis is a general mechanism in the biosynthesis of hormones, receptors and viral protein precursors. This proceeds by cleavage of peptide bonds at theExpand
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Immunization procedures forE. coli proteins
Three immunization procedures were compared for the production of antibodies to the minor components of a complexE. coli protein (ECP) mixture: a conventional protocol and two methods that allow forExpand
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Conformational and biological properties of the Ala10 analogue of human des-Trp1,Nle12-minigastrin.
Synthesis, conformation, and biological properties of the Ala10 analogue of des-Trp1,Nle12-minigastrin are reported. Replacement of the Gly residue in the original sequence with Ala remarkablyExpand
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Prosomatostatin processing in Neuro2A cells
Proline residues located near the processing sites of human prosomatostatin were previously shown to be important for cleavage of the precursor into somatostatin 28 and somatostatin 14 [Gomez, S.,Expand
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NMR conformational studies on a synthetic peptide reproducing the [1-20] processing domain of the pro-ocytocin-neurophysin precursor.
The combined use of several nuclear magnetic resonance and restrained molecular dynamics techniques allowed the formulation of a molecular model for the preferred solution conformation of a syntheticExpand
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Favourable side-chain orientation of cleavage site dibasic residues of prohormone in proteolytic processing by prohormone convertase 1/3.
Previous studies using selectively modified pro-ocytocin/neurophysin substrate analogues and the purified metalloprotease, pro-ocytocin/neurophysin convertase (magnolysin; EC 3.4 24.62), have shownExpand
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CD conformational studies on synthetic peptides encompassing the processing domain of the ocytocin/neurophysin precursor.
Synthetic peptides of different size, reproducing the proteolytic processing site of proocytocin, were studied by CD under several experimental conditions in order to ascertain the ability ofExpand
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Conformational studies on synthetic peptides reproducing the dibasic processing site of pro‐ocytocin–neurophysin
Synthetic peptides reproducing the proteolytic processing site of pro‐ocytocin were studied by different spectroscopic techniques, including circular dichroism, Fourier tranform infrared absorption,Expand
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New Fourier transform infrared based computational method for peptide secondary structure determination. I. Description of method.
Fourier transform infrared (FTIR) experiments in dimethylsulfoxide, a solvent incapable of H donation, demonstrate that H --> D isotopic replacement on the amide side of peptide bonds involvesExpand
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Synthesis, Characterization and Conformational Analysis of gp120‐derived Synthetic Peptides That Specifically Enhance HIV‐1 Infectivity
A series of peptides patterned on the principal neutralizing domain of the HIV‐1 envelope glycoprotein gp120 have been synthesized by solid‐phase techniques. Interestingly, in vitro experiments haveExpand
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