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cDNA microarray analysis of small plant tissue samples using a cDNA tag target amplification protocol.
Microarray technology is becoming an important comprehensive tool to study gene expression in plants. However, the use of this technology is limited by the large amount of sample tissue needed forExpand
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Comparative Immunization Study Using RNA and DNA Constructs Encoding a Part of the Plasmodium falciparum Antigen Pf332
Development of nucleic acid‐based vaccines against parasitic diseases shows great promise, although certain concerns about safety aspects of conventional DNA vaccines have been raised. This studyExpand
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Epidermal growth factor (EGF) withdrawal masks gene expression differences in the study of pituitary adenylate cyclase-activating polypeptide (PACAP) activation of primary neural stem cell
BackgroundThe recently discovered adult neural stem cells, which maintain continuous generation of new neuronal and glial cells throughout adulthood, are a promising and expandable source of cellsExpand
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Improving reliability and performance of DNA microarrays
A great many platforms and versions of the microarray technology, with different characteristics and applications, have been developed. This review will describe some key issues in reliability andExpand
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Gene expression analysis by signature pyrosequencing.
We describe a novel method for transcript profiling based on high-throughput parallel sequencing of signature tags using a non-gel-based microtiter plate format. The method relies on theExpand
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Amplification of mRNA populations by a cDNA tag strategy.
Here we describe an amplification method for global transcript analysis. The strategy relies on amplification of cDNA tags (signature tags) achieved by random fragmentation of the cDNAs to short tagsExpand
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Transcriptome analysis in primary neural stem cells using a tag cDNA amplification method
BackgroundNeural stem cells (NSCs) can be isolated from the adult mammalian brain and expanded in culture, in the form of cellular aggregates called neurospheres. Neurospheres provide an in vitroExpand
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Synthesis of fluorescent derivatives of 7-methylguanine through reaction with 2-aryl-substituted malondialdehydes: analysis by HPLC with fluorescence detection.
Fluorescent derivatives of 7-methylguanine were prepared through reaction with 2-aryl-substituted-malondialdehydes and analysed by reversed-phase HPLC with fluorescence detection. Reaction of carbonsExpand
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Transcript profiles and genotyping of cancer tissue
Molecular cloning of a cDNA encoding a cytosolic form of phosphoglucomutase from cambium of poplar (Populus tremula x tremuloides)
Molecular cloning of a cDNA encoding a cytosolic form of phosphoglucomutase from cambium of poplar (Populus tremula x tremuloides)