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A Simple Method for DNA Extraction from Mature Date Palm Leaves: Impact of Sand Grinding and Composition of Lysis Buffer
- I. Arif, M. A. Bakir, M. Shobrak
- Biology, MedicineInternational journal of molecular sciences
- 8 September 2010
This study suggested that grinding of date palm leaf with sterile sand and inclusion of NaCl in the lysis buffer without the costly use of liquid nitrogen, PVP and LiCl, provides a DNA yield of sufficient purity, suitable for PCR amplification.
A Brief Review of Molecular Techniques to Assess Plant Diversity
This review presents a basic description of different molecular techniques that can be utilized for DNA fingerprinting and molecular diversity analysis of plant species.
Interpretation of electrophoretograms of seven microsatellite loci to determine the genetic diversity of the Arabian Oryx.
This work interpreted electrophoretograms of seven microsatellite loci to determine the genetic diversity of the Arabian Oryx and suggests that correct identification of alleles in the presence of co-amplified nonspecific fragments is important for reliable interpretation of micros satellite data.
DNA marker technology for wildlife conservation.
Application of RAPD for molecular characterization of plant species of medicinal value from an arid environment.
- I. Arif, M. A. Bakir, M. Shobrak
- Biology, MedicineGenetics and molecular research : GMR
- 9 November 2010
The RAPD method successfully discriminates among all the plant species, therefore providing an easy and rapid tool for identification, conservation and sustainable use of these plants.
Measuring the genetic diversity of Arabian Oryx using microsatellite markers: implication for captive breeding.
- I. Arif, H. Khan, M. Shobrak, A. Homaidan, M. Sadoon, A. Farhan
- Biology, Environmental ScienceGenes & genetic systems
- 1 April 2010
Information about the extent of heterozygosity, allelic diversity and inbreeding/outbreeding depression using microsatellite markers could be of potential relevance for the management of captive breeding programs for the conservation of Arabian oryx.
Cytochrome c oxidase subunit I barcoding of the green bee-eater (Merops orientalis).
- I. Arif, H. Khan, M. Shobrak, J. Williams
- BiologyGenetics and molecular research : GMR
- 21 October 2011
This is the first study reporting barcodes for M. orientalis, a group of near passerine birds in the family Meropidae, and GenBank included a COI barcode for only one species of bee-eater.
DNA Barcodes of Asian Houbara Bustard (Chlamydotis undulata macqueenii)
- I. Arif, H. Khan, Joseph B. Williams, M. Shobrak, Waad I. Arif
- BiologyInternational journal of molecular sciences
- 22 February 2012
This is the first study reporting the barcodes of Houbara Bustard and would be helpful in future molecular studies, particularly for the conservation of this threatened bird in Saudi Arabia.
Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals.
- I. Arif, H. Khan, M. Al Sadoon, M. Shobrak
- Biology, Environmental ScienceGenetics and molecular research : GMR
- 31 October 2011
An extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds is reported.
Pesticide poisoning in a free-ranging lappetfaced vulture (Torgos tracheliotus)
A case of exposure to insecticide in a lappet-faced vulture following an aerial insecticide spray operation is described, thus representing, to the author's knowledge, the first documented case of pesticide poisoning of a bird of prey in Saudi Arabia.