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Cell Surface Display of Organophosphorus Hydrolase Using Ice Nucleation Protein
A new anchor system based on the ice nucleation protein (InaV) from Pseudomonas syringae INA5 was developed for cell surface display of functional organophosphorus hydrolase (OPH). The activity andExpand
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Functional characterization of renal chloride channel, CLCN5, mutations associated with Dent'sJapan disease.
BACKGROUND The annual urinary screening of Japanese children above three years of age has identified a progressive renal tubular disorder characterized by low molecular weight proteinuria,Expand
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Simultaneous degradation of organophosphorus pesticides and p-nitrophenol by a genetically engineered Moraxella sp. with surface-expressed organophosphorus hydrolase.
Moraxella sp., a native soil organism that grows on p-nitrophenol (PNP), was genetically engineered for the simultaneous degradation of organophosphorus (OP) pesticides and p-nitrophenol (PNP). TheExpand
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A sensitive and robust method for quantification of intracellular short-chain coenzyme A esters.
A procedure for the analysis of short-chain intracellular coenzyme A (CoA) esters and adenine nucleotide pools in microbial cells is described. The simultaneous isolation of bacterial cells fromExpand
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Thermally triggered purification and immobilization of elastin–OPH fusions
A bifunctional fusion protein consisting of organophosphorus hydrolase (OPH) and elastin‐like polypeptide (ELP) was synthesized for the detoxification of organophosphorus compounds. ELPs undergo aExpand
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Two-Exon Skipping Due to a Point Mutation in p67-phox-Deficient Chronic Granulomatous Disease
HRONIC GRANULOMATOUS disease (CGD) is a rare inherited disorder whereby phagocytes cannot generate superoxide anion and its derivatives to other active oxygen species that may be used for killingExpand
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Cell Surface Display of Organophosphorus Hydrolase in Pseudomonasputida Using an Ice‐Nucleation Protein Anchor
A surface anchor system derived from the ice‐nucleation protein (INP) from Pseudomonas syringe was used to localize organophosphorus hydrolase (OPH) onto the surface of Pseudomonas putida KT2440.Expand
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Recruiting cytotoxic T cells to folate-receptor-positive cancer cells.
Herein, we describe the synthesis of a chemically defined anti-CD3 Fab-folate conjugate that targets cytotoxic T cells to folate receptor positive (FR+) tumors. The unnatural amino acidExpand
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Two-exon skipping due to a point mutation in p67-phox--deficient chronic granulomatous disease.
The cytosolic 67-kD protein in phagocytes (p67-phox) and B lymphocytes is one of essential components of the superoxide-generating system in these cells, and its defect causes an autosomal recessiveExpand
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Role of tRNA Orthogonality in an Expanded Genetic Code
We found that Methanocaldococcus jannaschii DSM2661 tyrosyl-tRNA synthetase (Mj E9RS), specifically evolved to charge its cognate tRNA with the unnatural amino acid p-acetylphenylalanine (pAcF) in E.Expand
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