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An unusual case of 'uncompetitive activation' by ascorbic acid: purification and kinetic properties of a myrosinase from Raphanus sativus seedlings.
Myrosinase (thioglucoside glucohydrolase; EC 3.2.3.1) is a plant enzyme that hydrolyses glucosinolates, principally to isothiocyanates. Myrosinase was purified to homogeneity in good yield fromExpand
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Substrate specificity of adrenocortical cytochrome P-450scc--I. Effect of structural modification of cholesterol side-chain on pregnenolone production.
Abstract Thirty four cholesterol analogues with modified side chain were chemically synthesized and incubated with a highly purified preparation of cytochrome P-450scc. It was shown that five to tenExpand
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Specificity of radioprotective and cytotoxic effects of cysteamine in HeLa S3 cells: generation of peroxide as the mechanism of paradoxical toxicity.
Cysteamine at 0.5-5 mM effectively kills ${\rm HeLa}\ {\rm S}_{3}$ cells, while it is much less toxic in higher concentration (30 mM). The toxicity develops gradually with time as peroxide generatesExpand
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X-irradiation-induced emesis in Suncus murinus.
X-irradiation-induced emesis was investigated in Suncus murinus, a house musk shrew. Whole body X-irradiation caused emesis, and the calculated ED50 value that induced emesis in 50% of animals wasExpand
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Cytochrome P-450 from bovine adrenocortical mitochondria: an enzyme for the side chain cleavage of cholesterol. I. Purification and properties.
  • M. Shikita, P. Hall
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 25 August 1973
Abstract One cytochrome P-450 has been purified from bovine adrenocortical mitochondria to near homogeneity. The enzyme catalyzes the conversion of cholesterol and 20α-hydroxycholesterol toExpand
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The stoichiometry of the conversion of cholesterol and hydroxycholesterols to pregnenolone (3beta-hydroxypregn-5-en-20-one) catalysed by adrenal cytochrome P-450.
  • M. Shikita, P. Hall
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 April 1974
The stoichiometry of the side-chain cleavage of cholesterol (cholest-5-en-3beta-ol), 20alpha-hydroxycholesterol (cholest-5-ene-3beta,20alpha-diol), and 20alpha,22-dihydroxycholesterolExpand
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Preparation of highly purified 3α- and 3β-hydroxysteroid dehydrogenases from Pseudomonas sp
Abstract A method is described for preparing highly purified 3α- and 3β-hydroxysteroid dehydrogenases (EC 1.1.1.50 and EC 1.1.1.145, respectively), essentially uncontaminated with one another, fromExpand
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Effects of recombinant human granulocyte colony-stimulating factor on the hematologic recovery and survival of irradiated mice.
We studied the effects of intraperitoneal injections of recombinant human granulocyte colony-stimulating factor (rhG-CSF) according to various administration schedules on the recovery of spleenExpand
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Intraclonal diversity of fibrosarcoma cells for the production of macrophage colony‐stimulating factor and granulocyte colony‐stimulating factor
A new cell line was established from fibrosarcoma that had spontaneously developed in a mouse. The cells were maintained growing in cultue for two years and constantly produced both macrophageExpand
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Cytochrome P-450 from Bovine Adrenocortical Mitochondria : an Enzyme for the Side Chain Cleavage of Cholesterol
One cytochrome P-450 has been purified from bovine adrenocortical mitochondria to near homogeneity. The enzyme catalyzes the conversion of cholesterol and 20~ hydroxycholesterol to pregnenolone (sideExpand
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