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Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
Axoplasm from the squid giant axon contains a soluble protein translocator that induces movement of microtubules on glass, latex beads on microtubules, and axoplasmic organelles on microtubules. WeExpand
Single particle tracking. Analysis of diffusion and flow in two-dimensional systems.
Analysis of the trajectories of small particles at high spatial and temporal resolution using video enhanced contrast microscopy provides a powerful approach to characterizing the mechanisms ofExpand
Local force and geometry sensing regulate cell functions
  • V. Vogel, M. Sheetz
  • Biology, Medicine
  • Nature Reviews Molecular Cell Biology
  • 1 April 2006
The shapes of eukaryotic cells and ultimately the organisms that they form are defined by cycles of mechanosensing, mechanotransduction and mechanoresponse. Local sensing of force or geometry isExpand
Extracellular Matrix Rigidity Causes Strengthening of Integrin–Cytoskeleton Linkages
To move forward, migrating cells must generate traction forces through surface receptors bound to extracellular matrix molecules coupled to a rigid structure. We investigated whether cells sample andExpand
Force Sensing by Mechanical Extension of the Src Family Kinase Substrate p130Cas
How physical force is sensed by cells and transduced into cellular signaling pathways is poorly understood. Previously, we showed that tyrosine phosphorylation of p130Cas (Cas) in a cytoskeletalExpand
The relationship between force and focal complex development
To adhere and migrate, cells must be capable of applying cytoskeletal force to the extracellular matrix (ECM) through integrin receptors. However, it is unclear if connections between integrins andExpand
Phosphatidylinositol 4,5-Bisphosphate Functions as a Second Messenger that Regulates Cytoskeleton–Plasma Membrane Adhesion
Binding interactions between the plasma membrane and the cytoskeleton define cell functions such as cell shape, formation of cell processes, cell movement, and endocytosis. Here we use opticalExpand
Talin depletion reveals independence of initial cell spreading from integrin activation and traction
Cell spreading, adhesion and remodelling of the extracellular matrix (ECM) involve bi-directional signalling and physical linkages between the ECM, integrins and the cell cytoskeleton. TheExpand
Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin
Responses to cholesterol depletion are often taken as evidence of a role for lipid rafts in cell function. Here, we show that depletion of cell cholesterol has global effects on cell and plasmaExpand
Stretching Single Talin Rod Molecules Activates Vinculin Binding
The molecular mechanism by which a mechanical stimulus is translated into a chemical response in biological systems is still unclear. We show that mechanical stretching of single cytoplasmic proteinsExpand