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Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.
Functional and structural characterization of the thermostable BsCel5A (Bacillus subtilis cellulase 5A) revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif, and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Expand
The repeat domain of the type III effector protein PthA shows a TPR‐like structure and undergoes conformational changes upon DNA interaction
The results point to a model in which the RD2 structure changes its compactness as it embraces the DNA with the polymorphic diresidues facing the interior of the superhelix oriented toward the nucleotide bases, which is in agreement with the elongated shape of RD2 determined by SAXS. Expand
Functional diversification of cerato-platanins in Moniliophthora perniciosa as seen by differential expression and protein function specialization.
Cerato-platanins (CP) are small, cysteine-rich fungal-secreted proteins involved in the various stages of the host-fungus interaction process, acting as phytotoxins, elicitors, and allergens. WeExpand
How C-terminal carboxyamidation alters the biological activity of peptides from the venom of the eumenine solitary wasp.
The diverse biological activity of the two peptides is not simply due to the reduction of the net positive charge, as generally suggested, but also to a structural perturbation of the amphipathic alpha-helix that affects their ability to perturb the cell membrane. Expand
FtsZ filament capping by MciZ, a developmental regulator of bacterial division
Capping by MciZ makes FtsZ filaments shorter than normal, likely by blocking filament annealing; this represents fundamental information to understand how Fts Z filaments grow and shrink, and attain their normal size. Expand
Structure, dynamics, and RNA interaction analysis of the human SBDS protein.
The solution structure and backbone dynamics of the human SBDS protein are determined and its RNA binding site is described using NMR spectroscopy, which identifies a classicRNA binding site at the N-terminal FYSH domain that concentrates most of the mutations described for the humanSBDS. Expand
A Redox 2-Cys Mechanism Regulates the Catalytic Activity of Divergent Cyclophilins1[W]
It is shown that the conserved Cys residues form a disulfide bond that inactivates the enzyme, whereas Glu-83, which belongs to the catalytic loop and is also critical for enzyme activity, is anchored to the divergent loop to maintain the active site open. Expand
The solution structure of the outer membrane lipoprotein OmlA from Xanthomonas axonopodis pv. citri reveals a protein fold implicated in protein–protein interaction
The structure of OmlA does suggest that this protein may be implicated in protein–protein interactions required during X. citri infection, and its conformational and dynamic features were determined by nuclear magnetic resonance. Expand
Unsaturated fatty acids as high-affinity ligands of the C-terminal Per-ARNT-Sim domain from the Hypoxia-inducible factor 3α
Novel evidence is reported showing that unsaturated fatty acids are naturally occurring, non-covalent structural ligands of HIF-3α, thus providing the initial framework for exploring its exceptional role as a lipid sensor under hypoxia. Expand
Structural Analysis of Intermolecular Interactions in the Kinesin Adaptor Complex Fasciculation and Elongation Protein Zeta 1/ Short Coiled-Coil Protein (FEZ1/SCOCO)
The structural aspects of intermolecular interactions involved in this complex formation are investigated by NMR, cross-linking coupled with mass spectrometry (MS), SAXS (Small Angle X-ray Scattering) and molecular modelling, and it is proposed that homodimerization occurs with FEZ1 chains oriented in an anti-parallel topology. Expand