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Enzymology of the repair of free radicals-induced DNA damage
This review is focused on the repair of lesions induced by free radicals and ionising radiation and the BER pathway removes most of these DNA lesions, although recently it was shown that other pathways would also be efficient in the removal of oxidised bases. Expand
Requirement of mismatch repair genes MSH2 and MSH3 in the RAD1-RAD10 pathway of mitotic recombination in Saccharomyces cerevisiae.
It is shown that the mismatch repair genes MSH2 and MSH3 function in mitotic recombination, and studies presented here suggest an involvement of the RAD1-RAD10 pathway in reciprocal recombination. Expand
Alternative nucleotide incision repair pathway for oxidative DNA damage
It is shown that Nfo-like endonucleases nick DNA on the 5′ side of various oxidatively damaged bases, generating 3′-hydroxyl and 5′-phosphate termini, which provide the proper end for DNA repair synthesis. Expand
3,N4-ethenocytosine, a highly mutagenic adduct, is a primary substrate for Escherichia coli double-stranded uracil-DNA glycosylase and human mismatch-specific thymine-DNA glycosylase.
  • M. Saparbaev, J. Laval
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 21 July 1998
The fact that epsilonC is recognized and efficiently excised by the E. coli dsUDG and hTDG proteins in vitro suggests that these enzymes may be responsible for the repair of this mutagenic lesion in vivo and be important contributors to genetic stability. Expand
The major human AP endonuclease (Ape1) is involved in the nucleotide incision repair pathway.
It is reported that Ape1, the major apurinic/apyrimidinic endonuclease in human cells, is the damage- specific endonUClease involved in NIR, and the kinetic constants indicate that APe1-catalysed NIR activity is highly efficient. Expand
Psoralen-induced DNA adducts are substrates for the base excision repair pathway in human cells
It is demonstrated that bacterial homologues of NEIL1, the FPG and Nei proteins, also excise MAs, and new substrate specificity of the Fpg/Nei protein family provides an alternative repair pathway for ICLs and bulky DNA damage. Expand
1,N 2-Ethenoguanine, a Mutagenic DNA Adduct, Is a Primary Substrate of Escherichia coliMismatch-specific Uracil-DNA Glycosylase and Human Alkylpurine-DNA-N-Glycosylase*
It is reported that two structurally unrelated proteins, the Escherichia coli mismatch-specific uracil-DNA glycosylase (MUG) and the human alkylpurine-DNA-N-glycosylases (ANPG), can release 1,N 2-εG from defined oligonucleotides containing a single modified base. Expand
Apoptotic Topoisomerase I-DNA Complexes Induced by Staurosporine-mediated Oxygen Radicals*
It is proposed that Top1 cleavage complexes resulting from oxidative DNA lesions generated by ROS in staurosporine-treated cells contribute to the full apoptotic response. Expand
Uracil in duplex DNA is a substrate for the nucleotide incision repair pathway in human cells
The major human apurinic/apyrimidinic endonuclease, APE1, is a deoxyuridine end onuclease and can remove uracil residues in the DNA glycosylase-independent nucleotide incision repair pathway, pointing to a possible evolutionary origin of the DNA repair mechanisms for spontaneous damage to DNA in a common ancestor to all living forms. Expand
Antimutagenic role of base-excision repair enzymes upon free radical-induced DNA damage.
The various glycosylases involved in the repair of oxidised bases in Escherichia coli are reviewed and the Nfo protein was shown to have a novel activity that incises 5' to an alpha-deoxyadenosine residue (the anomer of deoxy adenosine formed by gamma-irradiation). Expand