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Hydroxycinnamic Acids in Walls of Wheat Aleurone Cells
Walls were isolated from sheets of aleurone cells from wheat, freed from associated protein and starch by extraction with phenol-acetic acid-water followed by treatment with porcine pancreatic alpha…
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Purification and characterization of an extracellular (1 --> 6)-beta-glucanase from the filamentous fungus Acremonium persicinum.
TLDR
An endo-(1 --> 6)-beta-glucanase has been isolated from the culture filtrates of the filamentous fungus Acremonium persicinum and purified by (NH4)2SO4 precipitation followed by anion-exchange and gel-filtration chromatography, indicating that the enzyme acts with retention of configuration.
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Determination of the solution structure of neuropeptide K by high-resolution nuclear magnetic resonance spectroscopy.
TLDR
A comparison of the tail region of NPK with the related peptide homologue, neurokinin A, in the same solvent system, indicates that both show increasing order when trifluoroethanol is titrated into water solution, with the appearance of sequential NOEs between backbone amide protons.
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Stereochemical course of glucan hydrolysis by barley (1-->3)- and (1-->3, 1-->4)-beta-glucanases.
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Conformational energy calculations and electrostatic potentials of dihydrofolate reductase ligands: relevance to mode of binding and species specificity.
TLDR
It is shown that the conformational flexibility of the connecting chain between the benzoyl glutamate and pteridine moieties in the folates actually allows the p teridine ring to shift between these alternative binding modes, a combination of which may offer the best explanation for the observed activity.
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Carbon-13 substituent chemical shifts in the side-chain carbons of aromatic systems: the importance of π-polarization in determining chemical shifts
13 C Substituent chemical shifts of the carbonyl sites in the side-chains of meta- and para-substituted benzenes of the type XC6H4COZ have been measured. Analysis of this data using the dual…
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Comparative review of molecular modelling software for personal computers
TLDR
This comparative assessment of molecular modelling software available for PCs will provide valuable guidance for investment in such software, both in the laboratory and at home.
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Electrosynthesis and solution structure of six-electron reduced forms of metatungstate, [H2W12O40]6−
Metatungstate salts α-[R4N]5H[H2W12O40] (R = Pr or Bu) were produced by phase transfer methods. Two one-electron reduction processes (E1/2 −1100, −1590 mV versus Ag–AgCl (saturated KCl in MeCN)) are…
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Conformational analysis of cyproheptadine hydrochloride.
TLDR
In the 1H NMR spectrum of 1 in CDCl3, two conformational forms are observed to occur in an approximate ratio of 1:4, and NOE and coupling constant measurements suggested that the terminal N-methyl group is equatorial.
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Comparison of native and mutant proteins provides a sequence-specific assignment of the cysteinyl ligand proton NMR resonances in the 2[Fe4S4] ferredoxin from Clostridium pasteurianum.
A sequence-specific assignment is presented for the eight low-field paramagnetically shifted cysteinyl ligand proton NMR resonances in the 2[Fe4S4] ferredoxin from Clostridium pasteurianum. The…
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